| dc.creator | Cervino, Vincenza | |
| dc.creator | Benaím, Gustavo | |
| dc.creator | Carafoli, Ernesto | |
| dc.creator | Guerini, Danilo | |
| dc.date | 2017-04-07T14:24:19Z | |
| dc.date | 2017-04-07T14:24:19Z | |
| dc.date | 1998 | |
| dc.date.accessioned | 2022-10-28T01:24:21Z | |
| dc.date.available | 2022-10-28T01:24:21Z | |
| dc.identifier | 1083-351X | |
| dc.identifier | http://hdl.handle.net/10872/15643 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4949205 | |
| dc.description | The effect of ethanol has been studied on four different isoforms of the plasma membrane Ca2+-ATPase expressed in Sf9 cells with the help of the baculovirus system. The PMCA2CI protein was maximally activated by 0.5% ethanol, a concentration 8–10 times lower than that needed to obtain the same effect on the PMCA4 protein or on the pump of erythrocyte membranes, which is a mixture of isoforms 1 and 4. Experiments performed with truncated pumps indicated that the stimulation by ethanol was lost if the C-terminal region between Lys1065 and Lys1161, encompassing the Calmodulin binding domain, was removed. These observations indicate that the stimulation is the result of a direct interaction of ethanol with the C-terminal regulatory domain of the Ca2+ pump. | |
| dc.language | en | |
| dc.publisher | THE JOURNAL OF BIOLOGICAL CHEMISTRY | |
| dc.relation | Vol. 273;No. 45 pp. 29811–29815 | |
| dc.subject | effect of ethanol | |
| dc.subject | isoforms | |
| dc.subject | plasma membrane | |
| dc.subject | Ca2+ | |
| dc.subject | ATPase | |
| dc.subject | baculovirus system | |
| dc.subject | ethanol | |
| dc.subject | PMCA4 protein | |
| dc.title | The Effect of Ethanol on the Plasma Membrane Calcium Pump Is Isoform-specific | |
| dc.type | Article | |
