Increased peroxide production by polymorphonuclear cells of chronic hepatitis C virus-infected patients.

dc.creatorToro, Felix
dc.creatorConesa, Angela
dc.creatorGarcia, Alexis
dc.creatorBianco Colmenares, Nicolás E.
dc.creatorDe Sanctis, Juan B.
dc.date2017-03-06T19:06:56Z
dc.date2017-03-06T19:06:56Z
dc.date1998-08
dc.date.accessioned2022-10-28T01:22:14Z
dc.date.available2022-10-28T01:22:14Z
dc.identifier0090-1229
dc.identifierhttp://hdl.handle.net/10872/14870
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4948466
dc.descriptionTo evaluate the oxidative burst in hepatitis C virus (HCV) infection, intracellular hydrogen peroxide (H202) production of polymorphonuclear (PMN) cells isolated from 15 chronic HCV-infected patients and 11 controls was assessed by flow cytometry in a time kinetic. Under nonstimulated and phorbol myristate acetate (PMA)-stimulated conditions, H202 production was higher in HCV-infected patients than in controls (P <0.05) at the time points of 20, 30, and 40 min. A positive correlation between H202 production by PMA-stimulated cells and serum levels of alanine aminotransferase and aspartate aminotransferase was found in the HCV-infected patients (r = 0.877, P <0.01 and r = 0.9351, P <0.001, respectively). RT-PCR analysis of purified mononuclear (MN) and PMN cells from HCV-infected patients revealed the presence of HCV RNA in 60% of MN and 27% of PMN cell samples. These results suggest that a functional alteration of PMN cells is manifested in this chronic viral infection which may represent an additional factor in the development of liver lesions.
dc.languageen
dc.publisherCLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY
dc.relationVol. 88;No. 2 pp 169-175
dc.subjectpolymorphonuclear cells
dc.subjecthepatitis C virus infection
dc.subjectperoxide production
dc.subjectflow cytometry
dc.subjectintracellular hydrogen
dc.subjectproduction of polymorphonuclear
dc.titleIncreased peroxide production by polymorphonuclear cells of chronic hepatitis C virus-infected patients.
dc.typeArticle


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