| dc.creator | Conesa, Angela | |
| dc.creator | Pérez, Carlos | |
| dc.creator | Rivera, Henry | |
| dc.creator | Aldrey, Oscar | |
| dc.creator | Bianco Colmenares, Nicolás E. | |
| dc.creator | De Sanctis, Juan B. | |
| dc.date | 2017-03-02T14:46:06Z | |
| dc.date | 2017-03-02T14:46:06Z | |
| dc.date | 1997 | |
| dc.date.accessioned | 2022-10-28T01:21:53Z | |
| dc.date.available | 2022-10-28T01:21:53Z | |
| dc.identifier | 0269-8951 | |
| dc.identifier | http://hdl.handle.net/10872/14717 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4948333 | |
| dc.description | A new method for normodense eosinophil purification is proposed. It is based on the use of high-density Ficoll-Hypaque (density = 1.114 g mL-1) instead of dextran sedimentation plus standard Ficoll-Hypaque, before discontinuous Percoll gradient centrifugation. Eosinophils from 11 controls and 11 eosinophilic patients were studied. The density distribution of eosinophils was similar in both groups. However, eosinophilic patients showed a significant increase in the absolute count of hypodense eosinophils. Normodense eosinophil purity and recovery were significantly higher in controls as compared with eosinophilic patients (p < 0.05). High-density Ficoll-Hypaque allowed separation of mononuclear and polymorphonuclear cells with high purity and recovery using a single centrifugation step. It also reduces one step in eosinophil purification as compared to the standard method. Thirdly, contrary to the single anti-CD16 magnetic bead separation method, it allows the isolation of normodense and hypodense eosinophils. In summary, this method is economic, reliable and permits the purification of different leucocyte populations. | |
| dc.language | en | |
| dc.publisher | Medical science research | |
| dc.relation | Vol. 25;No. 11 pp 751-755 | |
| dc.subject | normodense eosinophil | |
| dc.subject | high-density Ficoll-Hypaque | |
| dc.subject | eosinophil purification | |
| dc.subject | mononuclear | |
| dc.subject | polymorphonuclear cells | |
| dc.subject | anti-CD16 | |
| dc.subject | hypodense eosinophils | |
| dc.subject | leucocyte populations | |
| dc.title | High-density Ficoll-Hypaque as an alternative method of eosinophil purification | |
| dc.type | Article | |
