| dc.creator | DE SANCTIS, Juan B. | |
| dc.creator | BLANCA, Isaac | |
| dc.creator | Bianco Colmenares, Nicolás E. | |
| dc.date | 2017-02-21T20:14:45Z | |
| dc.date | 2017-02-21T20:14:45Z | |
| dc.date | 1995 | |
| dc.date.accessioned | 2022-10-28T01:21:25Z | |
| dc.date.available | 2022-10-28T01:21:25Z | |
| dc.identifier | 0143-5221 | |
| dc.identifier | http://hdl.handle.net/10872/14566 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4948170 | |
| dc.description | 1. T lymphocytes and large granular lymphocytes internalized chylomicrons, very low-density lipoprotein, low-density lipoprotein, high-density lipoprotein and acetyl modified low-density lipoprotein through different receptors as assessed by flow cytometry. The observed internalization ranged from 8% to 20%. 2. All lipoproteins induced proliferative responses in T lymphocytes and large granular lymphocytes at optimum concentrations (40 micrograms of protein/ml for all lipoproteins except high-density lipoprotein). Chylomicrons, very low-density lipoprotein and low-density lipoprotein increased T-lymphocyte proliferative response by fourfold while inducing respectively a seven-, nine- and sevenfold increment in large granular lymphocytes. Similarly, high-density lipoprotein and acetyl modified low-density lipoprotein respectively induced a nine- and sevenfold increment in T cells and a 17- and eightfold increment in large granular lymphocyte proliferative response. 3. Both cell types internalized more lipoprotein when they were stimulated with interleukin 2. Chylomicrons and low-density lipoprotein internalization was increased threefold and very low-density lipoprotein internalization twofold, while high-density lipoprotein internalization was unchanged in both cell types. Acetyl modified low-density lipoprotein internalization was fourfold higher in large granular lymphocytes only. 4. The proliferative response of interleukin-2 stimulated cells was different from that of unstimulated cells. Chylomicrons and very low-density lipoprotein induced a sixfold increment in T-cell proliferative response but only a fourfold increment in large granular lymphocytes. Low-density lipoprotein and acetyl modified low-density lipoprotein induced respectively a sevenfold and eightfold increment in T cells and a eightfold and threefold increment in large granular lymphocyte proliferative response. High-density lipoprotein did not affect T-lymphocyte proliferative response while inducing a twofold increase in large granular lymphocytes. 5. Lipoproteins are important in the proliferative response of unstimulated and interleukin-2-stimulated cells. | |
| dc.language | en | |
| dc.publisher | Clinical Science | |
| dc.relation | Vol. 89;No. 5 pp 511-519 | |
| dc.subject | chylomicron | |
| dc.subject | high-density lipoprotein | |
| dc.subject | interleukin-2 | |
| dc.subject | large granular Iymphocytes | |
| dc.subject | low-density lipoprotein | |
| dc.subject | T Iymphocytes | |
| dc.subject | very low-density lipoprotein | |
| dc.subject | flow cytometry | |
| dc.title | Effects of different lipoproteins on the proliferative response of interleukin-2-activated T lymphocytes and large granular lymphocytes | |
| dc.type | Article | |
