Anti-CD3-Activated T Cells from Chronic Nonviremic HBV Carriers Are Hyperreactive to Monocytic Accessory Signals

dc.creatorBAROJA, MIREN L.
dc.creatorSIRIT, FIRELEI L.
dc.creatorCALDERA, DILIAM J.
dc.creatorTORO, FELIX I.
dc.creatorZABALETA, MERCEDES E.
dc.creatorCOLMENARES, CARMEN J.
dc.creatorBIANCO COLMENARES, NICOLÁS E.
dc.creatorMACHADO, IRMA V.
dc.date2017-02-20T23:15:24Z
dc.date2017-02-20T23:15:24Z
dc.date1993
dc.date.accessioned2022-10-28T01:21:23Z
dc.date.available2022-10-28T01:21:23Z
dc.identifier0090-1229/93
dc.identifierhttp://hdl.handle.net/10872/14534
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4948158
dc.descriptionWe analyzed T cell responses through the CD3 activation pathway in a group of chronic HBV carriers. PBMC stimulated with the mAb OKT3 showed higher proliferative response in HBV-DNA(-) carriers compared to HBV-DNA(+) carriers and to controls. In contrast, no differences in proliferative responses were observed between HBV-DNA(-) carriers and controls in cell cultures stimulated with immobilized 64.1 mAb (SPB-64.1) which induces proliferation in the absence of monocytes. We further examined T cell responses in the presence of monocytes and their soluble factors to immobilized OKT3 mAb (SPB-OKT3). Purified T cells did not proliferate to SPB-OKT3. When autologous monocytes were added, higher proliferative response, IL-2 production, and IL-2 receptor expression were observed in HBV-DNA(-) carriers than in controls. An enhanced cell proliferation was also obtained when monocyte supernatants were added to T cells cultured with SPB-OKT3. Moreover, when IL-6 alone or combined with IL-1 was added to SPB-OKT3-stimulated T cell cultures, a significantly higher increase in T cell proliferation was detected in HBV-DNA(-) carriers. Our results thus show a T cell hyperreactivity to accessory signals from monocytes (mainly IL-6) in HBV-DNA(-) carriers, that is probably related to an ongoing viral clearance.
dc.languageen
dc.publisherCLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY
dc.relationVol. 69;No. 2 pp 180-188
dc.subjectAnti-CD3-Activated T Cells
dc.subjectChronic Nonviremic HBV Carriers
dc.subjectMonocytic Accessory
dc.subjectmAb OKT3
dc.subjectcell cultures stimulated
dc.subjectmonocytes
dc.subjectautologous monocytes
dc.subjectIL-6
dc.titleAnti-CD3-Activated T Cells from Chronic Nonviremic HBV Carriers Are Hyperreactive to Monocytic Accessory Signals
dc.typeArticle


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