| dc.creator | Salazar, Ana Maria | |
| dc.creator | Rodríguez-Acosta, Alexis | |
| dc.creator | Giron, Maria E | |
| dc.creator | Aguilar, Irma | |
| dc.creator | Guerrero, Belsy | |
| dc.date | 2013-11-27T17:28:42Z | |
| dc.date | 2013-11-27T17:28:42Z | |
| dc.date | 2013-11-27 | |
| dc.date.accessioned | 2022-10-28T00:56:23Z | |
| dc.date.available | 2022-10-28T00:56:23Z | |
| dc.identifier | http://hdl.handle.net/10872/5118 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4939341 | |
| dc.description | Venom constitution within the same snake species can present considerable
geographical variations. Bothrops atrox venoms were obtained from adult
snakes captured at different geographical locations: Parguasa (Bolı´var state); Puerto
Ayacucho 1, Serranı´a del Cuao and Puerto Ayacucho 2 (Amazon state). The coagulant
and fibrinolytic activities of these venoms were compared. Amidolytic activity of
crude snake venom was measured by a micromethod designed in our laboratory.
Coagulant activity on plasma and fibrinogen due to thrombin-like activity in venoms
was also determined. Crude snake venom fibrinolytic activity by the fibrin plate
method was assayed. Chromatographic studies were developed on Protein-Pack 300
column. Polyacrylamide gel electrophoresis was carried out under reduced
conditions. After SDS-PAGE of samples, the fibrin-zymography was tested on
agarose—fibrin plates.
The results demonstrated several differences among B. atrox venoms from
different geographical areas. Chromatograms and SDS-PAGE profiles indicated that
venoms from the same species presented differences in the molecular mass of their
components. The procoagulant activity depended on the utilized method (amidolytic
versus clotting). Parguasa and Puerto Ayacucho 2 venoms presented procoagulant
activity for both methods. Furthermore, Parguasa venom had also the highest
hemorrhagic activity and the lowest LD50. In relation to the fibrinolytic activity,
Puerto Ayacucho 1 venom was the most active, equally for fibrin plates as for the | |
| dc.description | This work was supported by FONACIT
G-2005000400 grant and IVIC grant. | |
| dc.language | en | |
| dc.relation | Thrombosis Research;120(1):95-104. (2007). | |
| dc.subject | Bothrops atrox | |
| dc.subject | Clotting activity | |
| dc.subject | Fibrinolytic activity | |
| dc.title | A comparative analysis of the clotting and fibrinolytic activities of the snake venom (Bothrops atrox) from different geographical areas in Venezuela | |
| dc.type | Article | |
