| dc.creator | Maita, Rosa | |
| dc.creator | Strauss, Miriam | |
| dc.creator | Anselmi, Guillermo | |
| dc.date | 2013-10-29T18:18:55Z | |
| dc.date | 2013-10-29T18:18:55Z | |
| dc.date | 2009-07-17 | |
| dc.date.accessioned | 2022-10-28T00:55:33Z | |
| dc.date.available | 2022-10-28T00:55:33Z | |
| dc.identifier | Volumen 22 | |
| dc.identifier | http://hdl.handle.net/10872/4657 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4938938 | |
| dc.description | In the present study, we compared the cell damage response in skeletal and cardiac muscle tissue when
exposed to doxorubicin. This was carried out by means of a less invasive informative substitute to endomyocardiac
biopsy based on Hsp70 immunodetection and a subcellular analysis of the nucleolus. Male Sprague Dawley rats (62 g
body weight) were randomly distributed into 3 group, the control and doxorubicin I and doxorubicin II groups, in which
15 and 25 mg/kg body weight of doxorubicin (0.1 ml, i.v.) was administered, respectively. After 15, 30, 45 and 60
minutes, portions of the left and right ventricle wall and interventricle wall, together with skeletal muscle from the
posterior and anterior member, were prepared for Hsp70 immunodetection by Western blot analysis and ultrastructural
study using the thin cut technique. Differential cell response between the control and treated groups was observed in
Hsp70 immunodetection and at the subcellular level. In the control group, the Hsp70 recognition levels and typical
normal nucleolar morphology were similar, while the treated groups showed variable-dependent Hsp70 recognition and
segregation of nucleolar components, forming ring-like figures of a variable-independent nature. Comparison of cardiac
and skeletal muscle tissue cell response to doxorubicin toxic aggression revealed parallelism in terms of Hsp70
accumulation in certain regions of both tissues (15 mg/kg body weight of doxorubicin), which suggests that replacing
endomyocardiac biopsy analysis with skeletal muscle analysis may be a safe option. | |
| dc.description | Comparison of cardiac and skeletal muscle tissue cell response to DOX toxic aggression revealed explicit similarity in terms of Hsp70 accumulation in certain regions
of both tissues, which suggests that the possibility of replacing endomyocardiac biopsy analysis with skeletal muscle analysis may be a safe option. | |
| dc.description | LOCTI-UCV(Laboratorios Elmor S.A.), Consejo de
Desarrollo Científico y Humanístico de la Universidad Central de Venezuela(CDCH-UCV). | |
| dc.language | en | |
| dc.publisher | Journal Toxicology and Pathology | |
| dc.subject | Doxorubicin | |
| dc.subject | Cardiomyopathy | |
| dc.subject | Endomyocardial Biopsy | |
| dc.subject | Skeletal Muscle | |
| dc.title | Skeletal Muscle for Endomyocardial Biopsy: Comparable Stress Response in Doxorubicin Cardio-myopathy | |
| dc.type | Article | |
