| dc.creator | Colina, C | |
| dc.creator | Flores, F | |
| dc.creator | Rojas, H | |
| dc.creator | Acosta, A | |
| dc.creator | Castillo, C | |
| dc.creator | Rosario, M | |
| dc.creator | Israel, A | |
| dc.creator | DiPolo, R | |
| dc.creator | Benaím, Gustavo | |
| dc.date | 2013-10-24T18:16:03Z | |
| dc.date | 2013-10-24T18:16:03Z | |
| dc.date | 2005-02 | |
| dc.date.accessioned | 2022-10-28T00:55:28Z | |
| dc.date.available | 2022-10-28T00:55:28Z | |
| dc.identifier | Vol. 436, pp. 333–345 | |
| dc.identifier | 0003-9861 | |
| dc.identifier | doi:10.1016/j.abb.2005.02.014 | |
| dc.identifier | http://hdl.handle.net/10872/4623 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4938905 | |
| dc.description | The effect of ceramide on the cytoplasmic Ca2+ concentration ([Ca2+]i) varies depending on the cell type. We have found that in
Jurkat human T cells ceramide increases the [Ca2+]i from a thapsigargin-sensitive calcium pool and the subsequent activation of a
capacitative Ca2+ entry. This effect occurs both in the presence and in the absence of extracellular calcium. Addition of ceramine, a
non-hydrolysable analogue of ceramide, reproduced its effect on the [Ca2+]i ruling out that this is due to the conversion of ceramide
to sphingosine. The effect of ceramide was additive to that obtained by sphingosine, but not to the Jurkat T cells specific antibody
OKT3. However, different to the latter, ceramide do not induced an elevation of InsP3. The opening of a store operated Ca2+ channel
by ceramide was corroborated by experiments of Fura-2 quenching, using Mn2+ as a surrogate for Ca2+ and confirmed by
whole-cell recording patch clamp techniques. | |
| dc.language | en | |
| dc.publisher | Archives of Biochemistry and Biophysics | |
| dc.subject | Ceramide | |
| dc.subject | Calcium | |
| dc.subject | Ceramine | |
| dc.subject | Capacitative calcium entry channel | |
| dc.subject | InsP3 | |
| dc.subject | Jurkat T cells | |
| dc.title | Ceramide increase cytoplasmic Ca2+ concentration in Jurkat T cells by liberation of calcium from intracellular stores and activation of a store-operated calcium channel | |
| dc.type | Article | |
