dc.creator | Vitta, Y. | |
dc.creator | Moliner-Martínez, Y. | |
dc.creator | Campíns-Falcó, P. | |
dc.creator | Fernández, Alberto | |
dc.date | 2013-07-29T21:18:20Z | |
dc.date | 2013-07-29T21:18:20Z | |
dc.date | 2010 | |
dc.date.accessioned | 2022-10-28T00:54:27Z | |
dc.date.available | 2022-10-28T00:54:27Z | |
dc.identifier | 0039-9140 | |
dc.identifier | http://hdl.handle.net/10872/4060 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4938400 | |
dc.description | We report a new device for the estimation of the content of chlorophyll a pigment in water samples as an indicator of water quality. The extraction of the pigment from water was also optimized. 10mL of water was filtered through a nylon filter (45 mpore size and 13mmof diameter), after the chlorophylls were dissolved by immersing the filter in 1mL of a low non-hazardous solvent as ethanol. An in-valve in-tube SPME device coupled to capillary liquid chromatography with diode array detection was designed. A capillary column of 70cm in length (0.32mm i.d. coated with 5% diphenyl–95% polydimethylsiloxane, 3 m coating thickness) was used as the loop of the injection valve for preconcentration and a Zorbax SB C18 (SiO2-based) 150mm×0.5mm i.d., 5 m column (Agilent) was used as analytical column. The achieved detection limit was 0.05 gL−1 and the working range of concentrations was 0.1–1 gL−1. % RSD values between 2 and 11 were obtained. Chlorophyll a in several water matrices was determined with good results in presence of other pigments such as chlorophyll b, pheophytin a and pheophytin b. | |
dc.language | en_US | |
dc.publisher | Talanta | |
dc.relation | ;82 | |
dc.subject | Chlorophyll a | |
dc.subject | In-tube solid phase microextraction | |
dc.subject | (IT-SPME) | |
dc.subject | Capillary LC | |
dc.subject | Water quality | |
dc.title | An in-tube SPME device for the selective determination of chlorophyll a in aquatic systems | |
dc.type | Article | |