New promising Euphorbiaceae extracts with activity in human lymphocytes from primary cell cultures

dc.creatorLlanes-Coronel, Doris S.
dc.creatorGámez-Díaz, Laura Y.
dc.creatorQuintero-Suarez, Leidy P.
dc.creatorPáez, Lady J.
dc.creatorTorres, Fernando
dc.creatorEcheverri, Fernando
dc.creatorPonte-Sucre, Alicia
dc.creatorPatiño, Pablo J.
dc.creatorTrujillo-Vargas, Claudia M.
dc.date2012-08-02T14:54:25Z
dc.date2012-08-02T14:54:25Z
dc.date2012-08-02
dc.date.accessioned2022-10-28T00:50:26Z
dc.date.available2022-10-28T00:50:26Z
dc.identifier0892-3973 (print)
dc.identifier1532-2513 (electronic)
dc.identifierhttp://hdl.handle.net/10872/1686
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4936301
dc.descriptionCONTEXT: Euphorbiaceae plants exhibit anti-inflammatory and immunomodulatory properties. METHODS: We evaluated the activity of 14 extracts from seven Euphorbiaceae plants on primary immune cell cultures from healthy individuals. Peripheral blood mononuclear cells (PBMC) were exposed to the extracts w/o phytohaemagglutinin A or cycloheximide as agents that induce proliferation or apoptosis in PBMC, respectively. RESULTS: We found that five up to 14 Euphorbiaceae's extracts had the ability to modulate at least one of the immune parameters evaluated in this study. However, only the latex extracts of Euphorbia cotinifolia and Euphorbia tirucalli strongly induced both proliferation and apoptosis in PBMC. These extracts were further subfractioned by silica gel column chromatography. Two subfractions with enhanced activity in comparison to the crude extracts were obtained. Although these subfractions induced proliferation on both CD3(+) and CD3(-) cells, the most prominent effects were observed in the former subpopulation. Interestingly, the subfraction from E. tirucalli induced lymphocyte proliferation without the need of accessory cells; this ability was not inhibited by the carbohydrates d-galactose and α-Methyl-D-Mannopyranoside. CONCLUSIONS: Altogether, these results reveal the presence of novel candidates within the Euphorbia plants to induce proliferation and apoptosis in human lymphocytes, mainly in CD3(+) T cells.
dc.descriptionCOLCIENCIAS
dc.languageen_US
dc.relationImmunopharmacology and Immunotoxicology;33:279-90, 2011
dc.subjectNatural Products;
dc.subjectLymphoproliferation
dc.subjectImmunomodulation
dc.subjectCytotoxicity
dc.subjectApoptosis
dc.titleNew promising Euphorbiaceae extracts with activity in human lymphocytes from primary cell cultures
dc.typeArticle


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