| dc.description | All cornponents of the angiotensin (AII) system are present in
the central nervoüs system .(l ). AII receptors have.been characterized
in nembrane fracti0ns of maíll'ral ian brain (2).
Binding sites for drugs and neurotransrnilters ca! be local ized
ancl quantítated in brain by autoradiography using [3¡¡¡11"ott,,u
and computerized densitometry after in vitrg^lncubat.lon with
appropriale ligands (3,4). T'he AII agonist 1251-¡5¿¡ll-AII js a
suitable l igand for AII recept0r sturlies since it shows complete
cr0ss displacsrient iljth All (5). These receptors have been recently
localized in discrete rat brain nuclei (6). Precise quantitation,
however, is essential for studies on the pllysiological re-
9ul atj on of receptors.
l.,le report tne quantitative deterlllination of AII rece¡Jtors jn.
.ar qrain, piLLitd.J ¿no acrenal glan,.: afler incubdLion vvitn -t¿5'l-
LSarl]-Al l. Tnis riernod jnvolves Llle use oi Jll-sensirive f il¡r fur
for auloradiography (4), selection of appropriate filn exp0sure
times, quantitation of optical densities bJ corlputerized microdensitornetry
(3) and cornparÍson to lzbl-stdnddrds iJrepared froln i:rain
ti ssue (7). | |
