Serologic diagnosis of human Taenia solium cysticercosis by using recombinant and synthetic antigens in QuickELISA

dc.date.accessioned2022-01-18T19:35:51Z
dc.date.available2022-01-18T19:35:51Z
dc.date.created2022-01-18T19:35:51Z
dc.date.issued2011
dc.identifierhttps://hdl.handle.net/20.500.12866/11212
dc.identifierhttps://doi.org/10.4269/ajtmh.2011.10-0079
dc.description.abstractDiagnosis of Taenia solium cysticercosis is an important component in the control and elimination of cysticercosis and taeniasis. New detection assays using recombinant and synthetic antigens originating from the lentil lectin-purified glycoproteins (LLGPs) of T. solium cysticerci were developed in a QuickELISA™ format. We analyzed a panel of 474 serum samples composed of 108 serum samples from donors with two or more viable cysts, 252 serum samples from persons with other parasitic infections, and 114 serum samples from persons with no documented illnesses. The sensitivities and specificities of T24H QuickELISA™, GP50 QuickELISA™, and Ts18var1 QuickELISA™ were 96.3% and 99.2%, 93.5% and 98.6%, and 89.8% and 96.4%, respectively, for detecting cases with multiple, viable cysts. T 24H QuickELISA™ performs best among the three assays, and has sensitivity and specificity values comparable to those of the LLGP enzyme-linked immunosorbent blot. The QuickELISA™ are simple, rapid quantitative methods for detecting antibodies specific for T. solium cysticerci antigens.
dc.languageeng
dc.publisherAmerican Society of Tropical Medicine and Hygiene
dc.relationAmerican Journal of Tropical Medicine and Hygiene
dc.relation1476-1645
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectHumans
dc.subjectcontrolled study
dc.subjectanimal cell
dc.subjectAnimals
dc.subjectnon nuclear magnetic resonance imaging
dc.subjectparasitology
dc.subjectparasite antigen
dc.subjectunclassified drug
dc.subjectmethodology
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectSensitivity and Specificity
dc.subjectimmunology
dc.subjectdiagnostic test accuracy study
dc.subjectSerologic Tests
dc.subjectserology
dc.subjectcomputer assisted tomography
dc.subjectcyst (resting stage)
dc.subjectenzyme linked immunosorbent assay
dc.subjectTaenia solium
dc.subjectCysticercosis
dc.subjectantibody detection
dc.subjectAntigens, Helminth
dc.subjectautoanalyzer
dc.subjectbaculovirus expression system
dc.subjectenzyme linked immunoelectrotransfer blot
dc.subjectglycoprotein
dc.subjecthelminth antibody
dc.subjecthelminth protein
dc.subjecthorseradish peroxidase
dc.subjectimmunoassay
dc.subjectLens culinaris
dc.subjectlentil lectin purified glycoprotein
dc.subjectquantitative assay
dc.subjectquick enzyme linked immunosorbent assay
dc.subjectrecombinant antigen
dc.subjectrecombinant antigen gp 50
dc.subjectrecombinant antigen sts 18 var1
dc.subjectrecombinant antigen t 24h
dc.subjectserodiagnosis
dc.subjectserum
dc.subjectstreptavidin
dc.titleSerologic diagnosis of human Taenia solium cysticercosis by using recombinant and synthetic antigens in QuickELISA
dc.typeinfo:eu-repo/semantics/article


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