Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children

dc.date.accessioned2022-01-18T19:26:52Z
dc.date.available2022-01-18T19:26:52Z
dc.date.created2022-01-18T19:26:52Z
dc.date.issued2012
dc.identifierhttps://hdl.handle.net/20.500.12866/10968
dc.identifierhttps://doi.org/10.1099/jmm.0.045443-0
dc.description.abstractThe aim of this study was to determine the frequency and allele associations of locus of enterocyte effacement encoded esp and tir genes among 181 enteropathogenic Escherichia coli (EPEC) strains (90 diarrhoea-associated and 91 controls) isolated from Peruvian children under 18 months of age. We analysed espA, espB, espD and tir alleles by PCR-RFLP. EPEC strains were isolated with higher frequency from healthy controls (91/424, 21.7 %) than from diarrhoeal samples (90/936, 9.6 %) (P<0.001); 28.9% of diarrhoeal and 17.6% of control samples were typical EPEC (tEPEC). The distribution of espA alleles (alpha, beta, beta2 and gamma) and espD alleles (alpha, beta, gamma and a new variant, espD-N1) between tEPEC and atypical EPEC (aEPEC) was significantly different (P<0.05). espD-alpha was more common among acute episodes (P<0.05). espB typing resulted in five alleles (alpha, beta, gamma and two new suballeles, espB-alpha2 and espB-alpha3), while tir-beta and tir-gamma2 were the most common intimin receptor subtypes. Seventy-two combinations of espA, espB, espD and tir alleles were found; the most prevalent combination was espA-beta, espB-beta, espD-beta, tir-beta (34/181 strains), which was more frequent among tEPEC strains (P,0.05). Our findings indicate that there is a high degree of heterogeneity among EPEC strains isolated from Peruvian children and that aEPEC and tEPEC variants cluster.
dc.languageeng
dc.publisherMicrobiology Society
dc.relationJournal of Medical Microbiology
dc.relation1473-5644
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectHumans
dc.subjectPeru
dc.subjectControlled Study
dc.subjectGenotype
dc.subjectNucleotide Sequence
dc.subjectInfant
dc.subjectGenetic Variation
dc.subjectDiarrhea
dc.subjectMolecular Sequence Data
dc.subjectPolymerase Chain Reaction
dc.subjectSequence Analysis DNA
dc.subjectGene Frequency
dc.subjectGenetic Association
dc.subjectDNA Bacterial
dc.subjectMolecular Epidemiology
dc.subjectPolymorphism Restriction Fragment Length
dc.subjectBacterial Gene
dc.subjectGene Locus
dc.subjectGenetic Variability
dc.subjectBacterium Isolation
dc.subjectEscherichia Coli Infections
dc.subjectPhosphoproteins
dc.subjectEnteropathogenic Escherichia Coli
dc.subjectEscherichia Coli Proteins
dc.subjectDNA Fingerprinting
dc.subjectEspa Alpha Gene
dc.subjectEspa Beta Gene
dc.subjectEspa Beta2 Gene
dc.subjectEspa Gamma Gene
dc.subjectEspb Alpha Gene
dc.subjectEspb Alpha2 Gene
dc.subjectEspb Alpha3 Gene
dc.subjectEspb Beta Gene
dc.subjectEspb Gamma Gene
dc.subjectEspd Alpha Gene
dc.subjectEspd Beta Gene
dc.subjectEspd Gamma Gene
dc.subjectEspd N1 Gene
dc.subjectMicrobial Diversity
dc.subjectTir Alpha Gene
dc.subjectTir Alpha2 Gene
dc.subjectTir Beta Gene
dc.subjectTir Gamma Gene
dc.subjectTir Gamma2 Gene
dc.titleGenetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children
dc.typeinfo:eu-repo/semantics/article


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