Identification of proteins associated with the formation of oral biofilms

dc.date.accessioned2021-10-04T23:00:58Z
dc.date.available2021-10-04T23:00:58Z
dc.date.created2021-10-04T23:00:58Z
dc.date.issued2021
dc.identifierhttps://hdl.handle.net/20.500.12866/9840
dc.identifierhttps://doi.org/10.1590/pboci.2021.084
dc.description.abstractObjective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion
dc.languageeng
dc.publisherAssociation of Support to Oral Health Research
dc.relationPesquisa Brasileira em Odontopediatria e Clinica Integrada
dc.relation1983-4632
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectacetone
dc.subjectadhesion
dc.subjectalcohol
dc.subjectArticle
dc.subjectbacterial growth
dc.subjectbacterial strain
dc.subjectbacterium culture
dc.subjectbiofilm
dc.subjectcarboxymethylcellulose
dc.subjectcentrifugation
dc.subjectcolony formation
dc.subjectcytoplasm protein
dc.subjectDental Plaque
dc.subjectdiaminobenzidine
dc.subjectelectrophoresis
dc.subjectFusobacterium nucleatum
dc.subjectgene amplification
dc.subjectgenomics
dc.subjectGenomics
dc.subjectglyceraldehyde 3 phosphate dehydrogenase (NADP)
dc.subjectglycerol
dc.subjectheat shock protein 40
dc.subjecthydrogen peroxide
dc.subjectnonhuman
dc.subjectoligonucleotide
dc.subjectphosphate buffered saline
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectpolymerase chain reaction
dc.subjectprotein analysis
dc.subjectprotein expression
dc.subjectprotein fingerprinting
dc.subjectpyroxylin
dc.subjectreal time polymerase chain reaction
dc.subjectsaliva substitute
dc.subjectStreptococcus gordonii
dc.subjecttooth plaque
dc.subjecttrypsin
dc.subjectultrasound
dc.subjectWestern blotting
dc.titleIdentification of proteins associated with the formation of oral biofilms
dc.typeinfo:eu-repo/semantics/article


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