In-depth analysis of a mixed Mycobacterium tuberculosis infection involving a multidrug-resistant strain and a susceptible strain

Abstract

Within a mycobacterial interspersed repetitive units–variable number tandem repeats (MIRU-VNTR) -based molecular epidemiology programme run on prisoners transferred from Peruvian prisons to a Spanish prison, we identified a mixed infection in one individual [[1]]. After segregating the two strains by serial dilutions on agar plates of the cultured isolates and single-colony analysis supported by MIRU-VNTR analysis, we detected the coexistence of a susceptible and a multidrug-resistant (MDR) strain (see Supplementary material, Table S1)