| dc.date.accessioned | 2019-12-06T21:04:48Z | |
| dc.date.available | 2019-12-06T21:04:48Z | |
| dc.date.created | 2019-12-06T21:04:48Z | |
| dc.date.issued | 2019 | |
| dc.identifier | https://hdl.handle.net/20.500.12866/7631 | |
| dc.identifier | https://doi.org/10.3390/app9122511 | |
| dc.description.abstract | The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 μm for PZA and 21 μm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA. | |
| dc.language | eng | |
| dc.publisher | MDPI | |
| dc.relation | Applied Sciences | |
| dc.relation | 2076-3417 | |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.subject | Drug resistant mycobacteria | |
| dc.subject | Lab-on-a-Chip (LoC) | |
| dc.subject | Personal medicine | |
| dc.subject | Quantitative SERS detection of PZA and POA | |
| dc.subject | Surface-enhanced Raman spectroscopy (SERS) | |
| dc.title | Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids | |
| dc.type | info:eu-repo/semantics/article | |
