dc.date.accessioned2019-02-06T14:57:38Z
dc.date.available2019-02-06T14:57:38Z
dc.date.created2019-02-06T14:57:38Z
dc.date.issued2015
dc.identifierhttps://hdl.handle.net/20.500.12866/5474
dc.identifierhttps://doi.org/10.4269/ajtmh.15-0232
dc.description.abstractLarge scale antibody responses in Plasmodium vivax malaria remains unexplored in the endemic setting. Protein microarray analysis of asexual-stage P. vivax was used to identify antigens recognized in sera from residents of hypoendemic Peruvian Amazon. Over 24 months, of 106 participants, 91 had two symptomatic P. vivax malaria episodes, 11 had three episodes, 3 had four episodes, and 1 had five episodes. Plasmodium vivax relapse was distinguished from reinfection by a merozoite surface protein-3alpha restriction fragment length polymorphism polymerase chain reaction (MSP3alpha PCR-RFLP) assay. Notably, P. vivax reinfection subjects did not have higher reactivity to the entire set of recognized P. vivax blood-stage antigens than relapse subjects, regardless of the number of malaria episodes. The most highly recognized P. vivax proteins were MSP 4, 7, 8, and 10 (PVX_003775, PVX_082650, PVX_097625, and PVX_114145); sexual-stage antigen s16 (PVX_000930); early transcribed membrane protein (PVX_090230); tryptophan-rich antigen (Pv-fam-a) (PVX_092995); apical merozoite antigen 1 (PVX_092275); and proteins of unknown function (PVX_081830, PVX_117680, PVX_118705, PVX_121935, PVX_097730, PVX_110935, PVX_115450, and PVX_082475). Genes encoding reactive proteins exhibited a significant enrichment of non-synonymous nucleotide variation, an observation suggesting immune selection. These data identify candidates for seroepidemiological tools to support malaria elimination efforts in P. vivax-endemic regions.
dc.languageeng
dc.publisherAmerican Society of Tropical Medicine and Hygiene
dc.relationAmerican Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.relation1476-1645
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAdolescent
dc.subjectAdult
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectYoung Adult
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectAged
dc.subjectAged, 80 and over
dc.subjectPolymerase Chain Reaction
dc.subjectRecurrence
dc.subjectPolymorphism, Single Nucleotide/genetics
dc.subjectAntibodies, Protozoan/immunology
dc.subjectAntibody Formation
dc.subjectAntigens, Protozoan/immunology
dc.subjectGene Expression/immunology
dc.subjectMalaria, Vivax/immunology
dc.subjectPlasmodium vivax/genetics/immunology
dc.subjectProtein Array Analysis
dc.titleGenome-Scale Protein Microarray Comparison of Human Antibody Responses in Plasmodium vivax Relapse and Reinfection
dc.typeinfo:eu-repo/semantics/article


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