dc.date.accessioned2019-02-06T14:48:07Z
dc.date.available2019-02-06T14:48:07Z
dc.date.created2019-02-06T14:48:07Z
dc.date.issued2016
dc.identifierhttps://hdl.handle.net/20.500.12866/5172
dc.identifierhttps://doi.org/10.1007/s11356-016-7537-9
dc.description.abstractThe objectives of the study were to detect and genotype Cryptosporidium spp. and Giardia intestinalis in wastewater samples obtained from five cities with high transit of people in the State of Sao Paulo, Brazil, and at the entrance of a Wastewater Treatment Plant (WWTP) in Lima, Peru. Samples were collected and concentrated by centrifugation. The genomic DNA was extracted for molecular characterization by nested PCR for Cryptosporidium and double nested PCR for Giardia, followed by sequencing and phylogenetic analysis. G. intestinalis was found in 63.6 % of the samples, and the human assemblages A and B were identified. Cryptosporidium sp. was found in 36.4 % of the samples, and the species were corresponding to Cryptosporidium hominis, Cryptosporidium cuniculus, and Cryptosporidium muris. Results revealed the presence of human pathogenic Cryptosporidium species and G. intestinalis human pathogenic assemblages. Molecular tools highlight the importance to map the genetic diversity of these parasites, as well as to detect their epidemiological circulation pathway in the environment.
dc.languageeng
dc.publisherSpringer
dc.relationEnvironmental Science and Pollution Research
dc.relation1614-7499
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCryptosporidium sp.
dc.subjectGiardia intestinalis
dc.subjectPCR
dc.subjectPublic health
dc.subjectWastewater
dc.subjectBrazil
dc.subjectCities
dc.subjectCryptosporidium/genetics/isolation & purification
dc.subjectGenotype
dc.subjectGiardia lamblia/genetics/isolation & purification
dc.subjectPeru
dc.subjectPhylogeny
dc.subjectPolymerase Chain Reaction
dc.subjectRNA, Protozoan/genetics
dc.subjectRNA, Ribosomal, 18S/genetics
dc.subjectSequence Analysis, DNA
dc.subjectWaste Water/parasitology
dc.titleOccurrence of Giardia intestinalis and Cryptosporidium sp in wastewater samples from Sao Paulo State, Brazil, and Lima, Peru
dc.typeinfo:eu-repo/semantics/article


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