The ichthyofauna of the upper Salí river is described from material collected through seasonal sampling. Considering highland and piain zones rivers, there were identified 25 species, including 16 new reports for this area and 4 new species for Tucumán province. The data were analized using association coefficients and cluster analysis. The species maximun density registered at each waterbody, ranges between 7 species at de Abajo river, and 14 at Salí river. The presence of Eigenmannia virescens adds to the endorreic basins of Argentina, a taxa belonging to a new reported order with Paranensean distribution.Facultad de Ciencias Naturales y Museo