Caracterización de las comunidades microbianas en suelos cultivados con banano bajo sistemas de manejo orgánico y convencional

Abstract

Soil is a complex and heterogeneous habitat colonized by a great diversity of microorganisms. One of the main limitations when characterizing microbial communities is that culture-dependent classical methodologies do not allow to study the effects caused on them as a community by crop management and production. The differences and similarities between the microbial communities in soils cultivated with banana under the management systems: organic and conventional are described below. For this, five paired sampling sites (banana plantations) were selected, 0.25 g of rhizosphere soil were collected and the environmental DNA (metagenome) was extracted from these samples. Afterwards, fragments of the 16S rDNA (bacteria) and ITS region (fungi) rDNA were amplified by PCR and the electrophoresed using denaturing gradient gel electrophoresis (DGGE). Diversity indexes (richness, Shannon and equitability) were calculated and sample cluster analysis was coducted from DGGE profiles. The results showed no significant differences (p > 0.05) between the bacterial and fungal communities in organically and conventionally managed soils