| dc.description.abstract | The combination of cryoprotective penetrating agents (CPA), such as glycerol (GLY) and
dimethylformamide (DMF), has shown encouraging results in equine sperm cryosurvival.
In this sense, this research evaluated the cryoprotective effect of GLY, DMF, and its
combination: DMF-GLY, on equine sperm quality and cryosurvival. For this purpose,
twenty-four semen ejaculates were collected from four healthy, sexually adult Arabian
horses (6 ejaculates/stallion). Each ejaculate was initially diluted with BotusemenSpecial extender and then frozen with Botusemen-Gold extender supplemented with
GLY (5%), DMF (5%), and DMF-GLY (3%-3%) using static liquid nitrogen vapors. The
fresh and frozen-thawing sperm samples were evaluated for their kinematics variables
and membranes integrity using the CASA computerized system (SCA-Evolution-2018)
and the double fluorescent test (PI / PNA-FITC), respectively. The results showed that
DMF alone or combined with GLY (DMF-GLY) was more effective in protecting equine
spermatozoa and producing higher values (P< 0,05) of total (MT, %) and progressive (MP,
%) motilities, curvilinear velocity (VCL), the amplitude of lateral head displacement (ALH)
and beat-cross frequency (BCF) compared to GLY. The combination of DMF-GLY yielded
higher (P < 0.05) strain-line (VSL) and average path velocities (VAP), plasma membrane
integrity (IMP, %), and acrosomal membrane integrity (IMA, %) compared to DMF alone
or GLY alone. In conclusion, the combination of DMF-GLY supplemented to the
'Botusemen-Gold' non-synthetic-based extender, was more suitable for cryoprotecting
Arabian horse sperm based on higher kinematics and membrane integrity compared to
glycerol cryoprotectant agent. | |
