Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions

dc.creatorLópez, Silvina Marianela Yanil
dc.creatorBalatti, Pedro Alberto
dc.date2012
dc.date2014-10-02T13:29:41Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/40776
dc.identifierhttp://astonjournals.com/manuscripts/Vol2011/GEBJ-34_Vol2011.pdf
dc.descriptionThe aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
dc.descriptionFacultad de Ciencias Agrarias y Forestales
dc.formatapplication/pdf
dc.languagees
dc.rightshttp://creativecommons.org/licenses/by/3.0/
dc.rightsCreative Commons Attribution 3.0 Unported (CC BY 3.0)
dc.subjectCiencias Agrarias
dc.subjectinoculant
dc.subjectbacterial DNA
dc.subjectarticle
dc.subjectmolecular markers
dc.subjectrhizobia
dc.subjectartifact
dc.subjectbacterial strain
dc.subjectbacterium culture
dc.subjectbacterium identification
dc.subjectbacterium isolate
dc.subjectbacterium mutant
dc.subjectBradyrhizobium
dc.subjectBradyrhizobium elkanii
dc.subjectBradyrhizobium japonicum
dc.subjectcontrolled study
dc.subjectDNA fingerprinting
dc.subjectgene amplification
dc.subjectinoculation
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectsoybean
dc.subjectBacteria (microorganisms)
dc.subjectBradyrhizobiaceae
dc.subjectGlycine max
dc.titleClosely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
dc.typeArticulo
dc.typeArticulo


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