dc.creatorMartos, María A.
dc.creatorMartínez Vázquez, Francisco
dc.creatorBenassi, Fernando O.
dc.creatorHours, Roque Alberto
dc.date2009-05
dc.date2014-07-03T21:40:05Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/37520
dc.identifierhttp://www.scielo.br/pdf/babt/v52n3/v52n3a07.pdf
dc.identifierissn:1516-8913
dc.descriptionResponse surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be suitable to be used in food processing in order to reduce the production of methanol.
dc.descriptionFacultad de Ciencias Exactas
dc.formatapplication/pdf
dc.format567-572
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc/3.0/
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
dc.subjectCiencias Exactas
dc.subjectQuímica
dc.subjectAspergillus Niger
dc.subjectCitrus pectin
dc.subjectpectinases
dc.subjectPectinesterase
dc.subjectpolygalacturonase
dc.subjectresponse surface methodology
dc.titleProduction of pectinases by <i>A. niger</i>: Influence of fermentation conditions
dc.typeArticulo
dc.typeArticulo


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