Association between GNRHR, LHR and IGF1 polymorphisms and timing of puberty in male Angus cattle

dc.creatorLirón, Juan Pedro
dc.creatorPrando, Alberto José
dc.creatorFernández, María Elena
dc.creatorRipoli, María Verónica
dc.creatorRogberg Muñoz, Andrés
dc.creatorGoszczynski, Daniel Estanislao
dc.creatorPosik, Diego Manuel
dc.creatorPeral García, Pilar
dc.creatorBaldo, Andrés
dc.creatorGiovambattista, Guillermo
dc.date2012-04
dc.date2014-07-03T21:30:26Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/37519
dc.identifierhttp://www.biomedcentral.com/content/pdf/1471-2156-13-26.pdf
dc.identifierissn:1471-2156
dc.descriptionBackground: In bovines, there are significant differences within and among beef breeds in the time when bulls reach puberty. Although the timing of puberty is likely to be a multigenic trait, previous studies indicate that there may also be single genes that exert major effects on the timing of puberty within the general population. Despite its economic importance, there are not many SNPs or genetic markers associated with the age of puberty in male cattle. In the present work, we selected three candidate genes, <i>GNRHR</i>, <i>LHR</i> and <i>IGF1</i>, and associated their polymorphisms with the age of puberty in Angus male cattle. Results: After weaning, 276 Angus males were measured every month for weight (W), scrotal circumference (SC), sperm concentration (C) and percentage of motility (M). A total of 4 SNPs, two within <i>GNRHR</i>, one in <i>LHR</i> and one in <i>IGF1</i> were genotyped using the pyrosequencing technique. <i>IGF1-SnaBI SNP</i> was significant associated (P < 0.01) with age at SC 28 cm, but it were not associated with age at M 10% and C 50 million. Genotype CC exhibited an average age at SC 28 cm of 7 and 11 days higher than CT (p = 0.037) and TT (p = 0.012), respectively. This SNP explained 1.5% of the genetic variance of age of puberty at SC28. <i>LHR-I499L, GNRHR-SNP5</i> and <i>GNRHR-SNP6</i> were not associated with any of the measurements. However, <i>GNRHR</i> haplotypes showed a suggestive association with age at SC 28 cm. Conclusions: The findings presented here could support the hypothesis that IGF1 is a regulator of the arrival to puberty in male calves and is involved in the events that precede and initiate puberty in bull calves. Given that most studies in cattle, as well as in other mammals, were done in female, the present results are the first evidence of markers associated with age at puberty in male cattle.
dc.descriptionFacultad de Ciencias Veterinarias
dc.formatapplication/pdf
dc.languagees
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar/
dc.rightsCreative Commons Attribution 2.5 Argentina (CC BY 2.5)
dc.subjectCiencias Veterinarias
dc.subjectgonadorelin receptor
dc.subjectbovine
dc.subjectsomatomedin
dc.subjectgonadotropin releasing hormone receptor
dc.subjectcattle
dc.subjectinsulin-like growth factor 1
dc.subjectluteinizing hormone receptor
dc.subjectgene sequence
dc.subjectpolymorphism
dc.subjectgenetic variability
dc.subjectgenotype
dc.titleAssociation between GNRHR, LHR and IGF1 polymorphisms and timing of puberty in male Angus cattle
dc.typeArticulo
dc.typeArticulo


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