dc.creator | Ibañez, Lorena Itatí | |
dc.creator | Caldevilla, Cecilia Andrea | |
dc.creator | Paredes Rojas, Yesica Luciana | |
dc.creator | Mattion, Nora Marta | |
dc.date.accessioned | 2019-11-28T21:34:00Z | |
dc.date.accessioned | 2022-10-15T16:27:00Z | |
dc.date.available | 2019-11-28T21:34:00Z | |
dc.date.available | 2022-10-15T16:27:00Z | |
dc.date.created | 2019-11-28T21:34:00Z | |
dc.date.issued | 2018-03-14 | |
dc.identifier | Ibañez, Lorena Itatí; Caldevilla, Cecilia Andrea; Paredes Rojas, Yesica Luciana; Mattion, Nora Marta; Genetic and subunit vaccines based on the stem domain of the equine influenza hemagglutinin provide homosubtypic protection against heterologous strains; Elsevier; Vaccine; 36; 12; 14-3-2018; 1592-1598 | |
dc.identifier | 0264-410X | |
dc.identifier | http://hdl.handle.net/11336/90847 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4409053 | |
dc.description.abstract | H3N8 influenza virus strains have been associated with infectious disease in equine populations throughout the world. Although current vaccines for equine influenza stimulate a protective humoral immune response against the surface glycoproteins, disease in vaccinated horses has been frequently reported, probably due to poor induction of cross-reactive antibodies against non-matching strains. This work describes the performance of a recombinant protein vaccine expressed in prokaryotic cells (ΔHAp) and of a genetic vaccine (ΔHAe), both based on the conserved stem region of influenza hemagglutinin (HA) derived from A/equine/Argentina/1/93 (H3N8) virus. Sera from mice inoculated with these immunogens in different combinations and regimes presented reactivity in vitro against highly divergent influenza virus strains belonging to phylogenetic groups 1 and 2 (H1 and H3 subtypes, respectively), and conferred robust protection against a lethal challenge with both the homologous equine strain (100%) and the homosubtypic human strain A/Victoria/3/75 (H3N2) (70–100%). Animals vaccinated with the same antigens but challenged with the human strain A/PR/8/34 (H1N1), belonging to the phylogenetic group 1, were not protected (0–33%). Combination of protein and DNA immunogens showed higher reactivity to non-homologous strains than protein alone, although all vaccines were permissive for lung infection. | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.vaccine.2018.02.019 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X18301932 | |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | CONSERVED EPITOPES | |
dc.subject | CROSS-PROTECTION | |
dc.subject | EQUINE INFLUENZA VIRUS | |
dc.subject | HEMAGGLUTININ STEM REGION | |
dc.subject | LETHAL CHALLENGE | |
dc.title | Genetic and subunit vaccines based on the stem domain of the equine influenza hemagglutinin provide homosubtypic protection against heterologous strains | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |