dc.creatorIbañez, Lorena Itatí
dc.creatorCaldevilla, Cecilia Andrea
dc.creatorParedes Rojas, Yesica Luciana
dc.creatorMattion, Nora Marta
dc.date.accessioned2019-11-28T21:34:00Z
dc.date.accessioned2022-10-15T16:27:00Z
dc.date.available2019-11-28T21:34:00Z
dc.date.available2022-10-15T16:27:00Z
dc.date.created2019-11-28T21:34:00Z
dc.date.issued2018-03-14
dc.identifierIbañez, Lorena Itatí; Caldevilla, Cecilia Andrea; Paredes Rojas, Yesica Luciana; Mattion, Nora Marta; Genetic and subunit vaccines based on the stem domain of the equine influenza hemagglutinin provide homosubtypic protection against heterologous strains; Elsevier; Vaccine; 36; 12; 14-3-2018; 1592-1598
dc.identifier0264-410X
dc.identifierhttp://hdl.handle.net/11336/90847
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4409053
dc.description.abstractH3N8 influenza virus strains have been associated with infectious disease in equine populations throughout the world. Although current vaccines for equine influenza stimulate a protective humoral immune response against the surface glycoproteins, disease in vaccinated horses has been frequently reported, probably due to poor induction of cross-reactive antibodies against non-matching strains. This work describes the performance of a recombinant protein vaccine expressed in prokaryotic cells (ΔHAp) and of a genetic vaccine (ΔHAe), both based on the conserved stem region of influenza hemagglutinin (HA) derived from A/equine/Argentina/1/93 (H3N8) virus. Sera from mice inoculated with these immunogens in different combinations and regimes presented reactivity in vitro against highly divergent influenza virus strains belonging to phylogenetic groups 1 and 2 (H1 and H3 subtypes, respectively), and conferred robust protection against a lethal challenge with both the homologous equine strain (100%) and the homosubtypic human strain A/Victoria/3/75 (H3N2) (70–100%). Animals vaccinated with the same antigens but challenged with the human strain A/PR/8/34 (H1N1), belonging to the phylogenetic group 1, were not protected (0–33%). Combination of protein and DNA immunogens showed higher reactivity to non-homologous strains than protein alone, although all vaccines were permissive for lung infection.
dc.languageeng
dc.publisherElsevier
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.vaccine.2018.02.019
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X18301932
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCONSERVED EPITOPES
dc.subjectCROSS-PROTECTION
dc.subjectEQUINE INFLUENZA VIRUS
dc.subjectHEMAGGLUTININ STEM REGION
dc.subjectLETHAL CHALLENGE
dc.titleGenetic and subunit vaccines based on the stem domain of the equine influenza hemagglutinin provide homosubtypic protection against heterologous strains
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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