Genotyping and study of adherence-related genes of Streptococcus uberis isolates from bovine mastitis

dc.creatorFessia, Alumine Soledad
dc.creatorDieser, Silvana Andrea
dc.creatorRaspanti, Claudia Gabriela
dc.creatorOdierno, Liliana Mónica
dc.date.accessioned2020-09-02T16:18:34Z
dc.date.accessioned2022-10-15T16:23:23Z
dc.date.available2020-09-02T16:18:34Z
dc.date.available2022-10-15T16:23:23Z
dc.date.created2020-09-02T16:18:34Z
dc.date.issued2019-05
dc.identifierFessia, Alumine Soledad; Dieser, Silvana Andrea; Raspanti, Claudia Gabriela; Odierno, Liliana Mónica; Genotyping and study of adherence-related genes of Streptococcus uberis isolates from bovine mastitis; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 130; 5-2019; 295-301
dc.identifier0882-4010
dc.identifierhttp://hdl.handle.net/11336/113053
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4408663
dc.description.abstractThe aim of this study was to determine the presence, conservation, and distribution of 6 potential adherence genes and their relationship with diverse molecular types in 34 S. uberis isolated from bovine mastitis in Argentina. Pulsed-field gel electrophoresis (PFGE) typing with SmaI was performed. The PCR for the detection of each gene, scpA, acdA, fbp, lbp, lmb, and sua was standardized. Samples of the amplification products were purified and sequenced. The PFGE patterns revealed the high level of heterogeneity of S. uberis, with 26 types of PFGE patterns. A high prevalence of scpA, fbp, lbp, lmb and acdA genes (100%?97%) was detected, whereas 79.41% of S. uberis harbored the sua gene. A high degree of similarity in the nucleotide and amino acid sequences of the 6 genes was observed. Our results showed that all genes are conserved and are present in most S. uberis isolates despite the wide clonal heterogeneity detected. This is the first study reporting an analysis of prevalence, and nucleotides and amino acids sequences of the potential adherence genes scpA, acdA, fbp, lbp, and lmb from S. uberis strains versus reported GenBank sequences, S. uberis 0140J and S. uberis NZ01.
dc.languageeng
dc.publisherAcademic Press Ltd - Elsevier Science Ltd
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.micpath.2019.03.027
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0882401019302803
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBOVINE MASTITIS
dc.subjectGENE DETECTION
dc.subjectGENE SEQUENCING
dc.subjectMOLECULAR TYPING
dc.subjectSTREPTOCOCCUS UBERIS
dc.titleGenotyping and study of adherence-related genes of Streptococcus uberis isolates from bovine mastitis
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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