dc.creatorCepeda, Sabrina Belén
dc.creatorSandoval, Marisa Julia
dc.creatorCrescitelli, Maria Carla
dc.creatorRauschemberger, María Belén
dc.creatorMassheimer, Virginia Laura
dc.date.accessioned2021-09-30T13:42:59Z
dc.date.accessioned2022-10-15T16:10:47Z
dc.date.available2021-09-30T13:42:59Z
dc.date.available2022-10-15T16:10:47Z
dc.date.created2021-09-30T13:42:59Z
dc.date.issued2020-01-02
dc.identifierCepeda, Sabrina Belén; Sandoval, Marisa Julia; Crescitelli, Maria Carla; Rauschemberger, María Belén; Massheimer, Virginia Laura; The isoflavone genistein enhances osteoblastogenesis: signaling pathways involved; Springer; Journal of Physiology and Biochemistry; 76; 2-1-2020; 99-110
dc.identifier1138-7548
dc.identifierhttp://hdl.handle.net/11336/142054
dc.identifier1877-8755
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4407330
dc.description.abstractPhytoestrogens have been proposed as a natural therapy for prevention of bone loss. In this work, we studied the mechanism of action of genistein on osteoblast differentiation. Primary cell cultures of calvarial osteoblasts isolated from female Wistar rats were in vitro exposed to genistein. Osteoblast differentiation markers were measured. Genistein stimulated osteoblast migration (71–257% above control). An earlier upregulation of estrogen receptor alpha gene expression and an enhancement of mRNA levels of the Runt-related transcription factor 2 were detected after 3 days of culture. The isoflavone significantly increased osteocalcin expression, extracellular collagen deposition, and alkaline phosphatase activity. The mechanism displayed by genis- tein involved estrogen receptor and nitric oxide pathway participation, since cell preincubation with the estrogen receptor antagonist ICI 182780, or the nitric oxide synthase inhibitor L-NAME, suppressed the phytoestrogen action. Evidence of MAPK and PI3K transduction systems participation on the stimulatory action of genistein on extracellular collagen deposition and alkaline phosphatase activity was also obtained. Genistein favored monocyte adhesion to osteoblasts (77% above control) in an ER; NOS; and MAPK kinase–dependent and PI3K-dependent manner. Co-cultured osteoblast-monocyte long term exposed (21 days) to genistein exhibited a high number of multinucleated and tartrate-resistant acid phosphatase–positive cells added to osteoblasts, suggesting that the phytoestrogen promotes osteoclast differentiation. In conclusion, genistein promoted osteoblasto- genesis through the participation of ER and NOS pathways, and the contribution of ERK or PI3K signal transduction pathways, and also stimulates osteoclast differentiation from its mononuclear progenitor.
dc.languageeng
dc.publisherSpringer
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/ 10.1007/s13105-019-00722-3
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13105-019-00722-3
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectGENISTEIN
dc.subjectOSTEOBLASTS
dc.subjectOSTEOCLASTS
dc.subjectDIFFERENTIATION MARKERS
dc.subjectEXTRACELLULAR MATRIX
dc.titleThe isoflavone genistein enhances osteoblastogenesis: signaling pathways involved
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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