A colorimetric method for the assay of ADP-glucose pyrophosphorylase

dc.creatorFusari, Corina
dc.creatorDemonte, Ana María Magdalena
dc.creatorFigueroa, Carlos Maria
dc.creatorAleanzi, Mabel Cristina
dc.creatorIglesias, Alberto Alvaro
dc.date.accessioned2019-10-11T19:16:47Z
dc.date.accessioned2022-10-15T15:45:58Z
dc.date.available2019-10-11T19:16:47Z
dc.date.available2022-10-15T15:45:58Z
dc.date.created2019-10-11T19:16:47Z
dc.date.issued2006-05
dc.identifierFusari, Corina; Demonte, Ana María Magdalena; Figueroa, Carlos Maria; Aleanzi, Mabel Cristina; Iglesias, Alberto Alvaro; A colorimetric method for the assay of ADP-glucose pyrophosphorylase; Academic Press Inc Elsevier Science; Analytical Biochemistry; 352; 1; 5-2006; 145-147
dc.identifier0003-2697
dc.identifierhttp://hdl.handle.net/11336/85780
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4404762
dc.description.abstractThe purpose of the current work was to develop a relatively simple method to assay ADPGlcPPase activity having high sensitivity, accuracy, and reliability. We sought a procedure allowing the assay in both of the reaction directions, based on a technique suitable for the screening of numerous samples. The method quantifies inorganic orthophosphate released from the specific hydrolysis of the enzyme activity products. For ADPGlc synthesis, the method measures Pi after hydrolysis of PPi by inorganic pyrophosphatase. Pyrophosphorolysis is assayed by determining Pi derived from CF1-ATPase-mediated hydrolysis of ATP. Pi dosage is performed by the technique based in the formation of a phosphomolybdate?Malachite Green complex [14,15]. We optimized the procedure to a microscale grade, reaching convenient sensitivity and the possibility of automation, by using a microplate (multiwell) absorbance reader
dc.languageeng
dc.publisherAcademic Press Inc Elsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ab.2006.01.024
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectADP-GLUCOSE PYROPHOSPHORYLASE
dc.titleA colorimetric method for the assay of ADP-glucose pyrophosphorylase
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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