dc.creator | Consolini, Alicia Elvira | |
dc.creator | Ragone, María Inés | |
dc.creator | Bonazzola, Patricia | |
dc.date.accessioned | 2020-01-30T17:33:55Z | |
dc.date.accessioned | 2022-10-15T15:43:13Z | |
dc.date.available | 2020-01-30T17:33:55Z | |
dc.date.available | 2022-10-15T15:43:13Z | |
dc.date.created | 2020-01-30T17:33:55Z | |
dc.date.issued | 2011-07 | |
dc.identifier | Consolini, Alicia Elvira; Ragone, María Inés; Bonazzola, Patricia; Mitochondrial and cytosolic calcium in rat hearts under high-K+ cardioplegia and pyruvate: Mechano-energetic performance; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 89; 7; 7-2011; 485-496 | |
dc.identifier | 0008-4212 | |
dc.identifier | http://hdl.handle.net/11336/96234 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4404443 | |
dc.description.abstract | High-K+-cardioplegia (CPG) and pyruvate (Pyr) are used as cardioprotective agents. Considering that mitochondria play a critical role in cardiac dysfunction, we investigated the effect of CPG on mitochondrial Ca2+ uptake and sarcorreticular (SR) calcium handling. Cytosolic and mitochondrial Ca2+, as well as mitochondrial membrane potential (ΔΨm) were assessed in rat cardiomyocytes by confocal microscopy. Mechano-calorimetrical correlation was studied in perfused hearts. CPG did not modify JC-1 (ΔΨm), but transiently increased, by up to 1.8 times, the Fura-2 (intracellular Ca concentration, [Ca2+]i) and Rhod-2 (mitochondrial free Ca concentration [Ca2+]m) fluorescence of resting cells, with exponential decays. The addition of 5 µmol·L–1 thapsigargin (Tpg) increased the Rhod-2 fluorescence in a group of cells without any effect on the Fura-2 signal. In rat hearts perfused with CPG, 1 µmol·L–1 Tpg decreased resting heat rate (ΔHr: –0.44 ± 0.07 mW·g–1), while the addition of 5 µmol·L–1 KB-R7943 increased resting pressure (ΔrLVP by +5.26 ± 1.10 mm Hg; 1 mm Hg = 133.322 Pa). The addition of 10 mmol·L–1 Pyr to CPG increased Hr (+3.30 ± 0.24 mW·g–1) and ΔrLVP (+2.2 ± 0.4 mm Hg), which are effects potentiated by KB-R7943. The results suggest that under CPG, (i) there was an increase in [Ca2+]i and [Ca2+]m (without changing ΔΨm) that decayed by exothermic removal mechanisms; (ii) mitochondrial Ca2+ uptake contributed to the removal of cytosolic Ca2+, in a process that was potentiated by inhibition of sarco–endoplasmic reticulum Ca2+-ATPase (SERCA), and reduced by KB-R7943; (iii) under these conditions, SERCA represents the main energetic consumer; (iv) Pyr increased the energetic performance of hearts,mainly by inducing mitochondrial metabolism. | |
dc.language | eng | |
dc.publisher | National Research Council Canada-NRC Research Press | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.nrcresearchpress.com/doi/10.1139/y11-042#.XjMSpGhKivM | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1139/y11-042 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | CA2+ | |
dc.subject | CALORIMETRY | |
dc.subject | CARDIOMYOCYTES | |
dc.subject | CARDIOPLEGIA | |
dc.subject | FURA-2 | |
dc.subject | HEART | |
dc.subject | PYRUVATE | |
dc.subject | RHOD-2 | |
dc.title | Mitochondrial and cytosolic calcium in rat hearts under high-K+ cardioplegia and pyruvate: Mechano-energetic performance | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |