Detection of Neospora spp. antibodies in horses from La Pampa, Argentina

dc.creatorRojas, M. C.
dc.creatorMoré, Gastón Andrés
dc.creatorCampero, Lucía María
dc.creatorFort, M.
dc.creatorGiménez, H.
dc.creatorVenturini, M. C.
dc.date.accessioned2021-07-23T19:33:37Z
dc.date.accessioned2022-10-15T15:32:33Z
dc.date.available2021-07-23T19:33:37Z
dc.date.available2022-10-15T15:32:33Z
dc.date.created2021-07-23T19:33:37Z
dc.date.issued2016
dc.identifierDetection of Neospora spp. antibodies in horses from La Pampa, Argentina; 10th International Equine Infectious Diseases Conference; Ciudad Autónoma de Buenos Aires; Argentina; 2016; s50-s50
dc.identifier0737-0806
dc.identifierhttp://hdl.handle.net/11336/136822
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4403330
dc.description.abstractNeospora caninum and N. hughesi are apicomplexan parasitesaffecting several animal species worldwide. Horses have beenreported as intermediate hosts of these protozoans. Neosporacaninum is an important cause of bovine abortion and neurologicalsigns in dogs while N. hughesi is reported as potential cause ofequine protozoal myeloencephalitis (EPM). Little is known aboutneosporosis in Argentinean horses. The aim of the present studywas to indentify antibodies against Neospora spp. in horses fromLa Pampa, Argentina. Serum samples (n¼42) were randomlyselected from a group of 300 horses belonging to rural areas fromLa Pampa province, Argentina. All animals were clinically healthy.The samples were processed by 4 serological tests using N. caninumantigen: indirect ELISA (CIVTEST HIPRA) with G protein asconjugate; a competitive ELISA (VMRD) proceeding according tomanufacturer instructions; indirect fluorescence antibody test(IFAT) and Immunoblot (IB) both using Nc1 strain as antigen.Serum sample from an experimentally infected (Nc1 strain)young horse was used as positive control. Positive serologicalresults were detected as follows: 30% of samples (n¼13) by ELISAHIPRA,28% (n¼12) by IFAT, 26% (n¼11) by IB and 16% (n¼7) byELISA-VMRD. A total of 12 samples resulted positive to 2 or moretests. Most of the positive samples (n ¼ 9) were seropositive byIFAT and IB. Considering the positivity to these 2 tests as ?truepositive?, 21.4% of horses showed antibodies against N. caninumantigens. Since potential cross reactions between Neospora spp.are plausible we considered the detected antibodies as genusspecific.Further studies should be conducted with specific N.hughesi serological tests for specific differentiation of the immunologicalresponses observed. The present study is the first reportof Neospora spp. antibodies detection in Argentinean horses. Theseropositivity rate detected suggests that horses from La Pampahave a frequent contact with Neospora spp. Future studies will beconducted in horses with clinical signs.
dc.languageeng
dc.publisherElsevier
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0737080616001416
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.jevs.2016.02.110
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceJournal of Equine Veterinary Science
dc.subjectNEOSPORA SPP.
dc.subjectANTIBODIES
dc.subjectHORSES
dc.subjectLA PAMPA
dc.titleDetection of Neospora spp. antibodies in horses from La Pampa, Argentina
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeinfo:eu-repo/semantics/conferenceObject
dc.typeinfo:ar-repo/semantics/documento de conferencia


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