dc.creatorOresti, Gerardo Martin
dc.creatorSantiago Valtierra, Florencia Ximena
dc.creatorAveldaño, Marta Isabel
dc.date.accessioned2022-05-05T12:41:49Z
dc.date.accessioned2022-10-15T14:56:38Z
dc.date.available2022-05-05T12:41:49Z
dc.date.available2022-10-15T14:56:38Z
dc.date.created2022-05-05T12:41:49Z
dc.date.issued2019
dc.identifierBiosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells; The LV Annual SAIB Meeting and XIV PABMB Congress; Argentina; 2019; 34-34
dc.identifierhttp://hdl.handle.net/11336/156614
dc.identifier1667-5746
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4399428
dc.description.abstractThe sphingomyelins (SM) and ceramides (Cer) of rodent spermatogenic cells contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms. The SM and Cer species with n-V, present in meiotic spermatocytes, become in part h-V species in post-meiotic spermatids. In each of these cells, the mentioned species are located in the non-raft fraction of the plasma membrane. The enzymes required for PUFA elongation to n-V, Elovl5, Elovl2 and Elovl4, and the fatty acid 2-hydroxylase (Fa2h) that converts n-V to h-V, are expressed in germ cells, with Elovl4 and Fa2h protein levels being highest in spermatocytes and spermatids, respectively. The Cer and SM species with n-V and h-V are biosynthesized de novo in a germ cell type-specific and steroid hormone-dependent manner. CerS3, which specifically N-acylates VLCPUFA to sphinganine, is highly expressed in meiotic cells. The Elovl4 and CerS3 protein expression prevails in spermatocytes, is seminiferous stage-specific, and is mostly concomitant. In spermatids, the Fa2h protein appears concentrated in late stages, especially when they elongate and their heads change shape. The unique sphingolipid species with n-V and h-V, as well as the enzymes involved in their biosynthesis, are useful biomarkers for investigating normal and pathological aspects of germ and sperm cell functions. Supported by SGCyT UNS-PGI-UNS (24/B272 to GMO and 24/B218 to MIA), FONCyT (PICT2017-2535 to GMO).
dc.languageeng
dc.publisherTech Science Press
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdf
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiocell
dc.subjectVLCPUFA
dc.subjectSPHINGOLIPIDS
dc.subjectSPERM
dc.subjectGERM CELLS
dc.titleBiosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeinfo:eu-repo/semantics/conferenceObject
dc.typeinfo:ar-repo/semantics/documento de conferencia


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