dc.creator | Oresti, Gerardo Martin | |
dc.creator | Santiago Valtierra, Florencia Ximena | |
dc.creator | Aveldaño, Marta Isabel | |
dc.date.accessioned | 2022-05-05T12:41:49Z | |
dc.date.accessioned | 2022-10-15T14:56:38Z | |
dc.date.available | 2022-05-05T12:41:49Z | |
dc.date.available | 2022-10-15T14:56:38Z | |
dc.date.created | 2022-05-05T12:41:49Z | |
dc.date.issued | 2019 | |
dc.identifier | Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells; The LV Annual SAIB Meeting and XIV PABMB Congress; Argentina; 2019; 34-34 | |
dc.identifier | http://hdl.handle.net/11336/156614 | |
dc.identifier | 1667-5746 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4399428 | |
dc.description.abstract | The sphingomyelins (SM) and ceramides (Cer) of rodent spermatogenic cells contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms. The SM and Cer species with n-V, present in meiotic spermatocytes, become in part h-V species in post-meiotic spermatids. In each of these cells, the mentioned species are located in the non-raft fraction of the plasma membrane. The enzymes required for PUFA elongation to n-V, Elovl5, Elovl2 and Elovl4, and the fatty acid 2-hydroxylase (Fa2h) that converts n-V to h-V, are expressed in germ cells, with Elovl4 and Fa2h protein levels being highest in spermatocytes and spermatids, respectively. The Cer and SM species with n-V and h-V are biosynthesized de novo in a germ cell type-specific and steroid hormone-dependent manner. CerS3, which specifically N-acylates VLCPUFA to sphinganine, is highly expressed in meiotic cells. The Elovl4 and CerS3 protein expression prevails in spermatocytes, is seminiferous stage-specific, and is mostly concomitant. In spermatids, the Fa2h protein appears concentrated in late stages, especially when they elongate and their heads change shape. The unique sphingolipid species with n-V and h-V, as well as the enzymes involved in their biosynthesis, are useful biomarkers for investigating normal and pathological aspects of germ and sperm cell functions. Supported by SGCyT UNS-PGI-UNS (24/B272 to GMO and 24/B218 to MIA), FONCyT (PICT2017-2535 to GMO). | |
dc.language | eng | |
dc.publisher | Tech Science Press | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/ | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdf | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Biocell | |
dc.subject | VLCPUFA | |
dc.subject | SPHINGOLIPIDS | |
dc.subject | SPERM | |
dc.subject | GERM CELLS | |
dc.title | Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.type | info:eu-repo/semantics/conferenceObject | |
dc.type | info:ar-repo/semantics/documento de conferencia | |