Inhibition of recombinant aldose-6-phosphate reductase from peach leaves by hexose-phosphates, inorganic phosphate and oxidants

dc.creatorHartman, Matias Daniel
dc.creatorFigueroa, Carlos Maria
dc.creatorArias, Diego Gustavo
dc.creatorIglesias, Alberto Alvaro
dc.date.accessioned2018-12-14T14:25:28Z
dc.date.accessioned2022-10-15T13:31:36Z
dc.date.available2018-12-14T14:25:28Z
dc.date.available2022-10-15T13:31:36Z
dc.date.created2018-12-14T14:25:28Z
dc.date.issued2017-01
dc.identifierHartman, Matias Daniel; Figueroa, Carlos Maria; Arias, Diego Gustavo; Iglesias, Alberto Alvaro; Inhibition of recombinant aldose-6-phosphate reductase from peach leaves by hexose-phosphates, inorganic phosphate and oxidants; Oxford University Press; Plant And Cell Physiology; 58; 1; 1-2017; 145-155
dc.identifier0032-0781
dc.identifierhttp://hdl.handle.net/11336/66481
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4391627
dc.description.abstractGlucitol, also known as sorbitol, is a major photosynthetic product in plants from the Rosaceae family. This sugar alcohol is synthesized from glucose-6-phosphate by the combined activities of aldose-6-phosphate reductase (Ald6PRase) and glucitol-6-phosphatase. In this work we show the purification and characterization of recombinant Ald6PRase from peach leaves. The recombinant enzyme was inhibited by glucose-1-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate and orthophosphate. Oxidizing agents irreversibly inhibited the enzyme and produced protein precipitation. Enzyme thiolation with oxidized glutathione protected the enzyme from insolubilization caused by diamide, while incubation with NADP+ (one of the substrates) completely prevented enzyme precipitation. Our results suggest that Ald6PRase is finely regulated to control carbon partitioning in peach leaves.
dc.languageeng
dc.publisherOxford University Press
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1093/pcp/pcw180
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/pcp/article/58/1/145/2736391
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCARBON PARTITIONING
dc.subjectGLUCITOL
dc.subjectPEACH
dc.subjectPRUNUS PERSICA
dc.subjectREDOX REGULATION
dc.subjectSORBITOL
dc.titleInhibition of recombinant aldose-6-phosphate reductase from peach leaves by hexose-phosphates, inorganic phosphate and oxidants
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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