dc.creator | Pérez, Pablo Aníbal | |
dc.creator | Toledo, Jonathan | |
dc.creator | Sosa, Liliana del Valle | |
dc.creator | Peinetti, Nahuel | |
dc.creator | Torres, Alicia Ines | |
dc.creator | de Paul, Ana Lucia | |
dc.creator | Gutiérrez, Silvina | |
dc.date.accessioned | 2021-11-09T19:40:43Z | |
dc.date.accessioned | 2022-10-15T13:21:39Z | |
dc.date.available | 2021-11-09T19:40:43Z | |
dc.date.available | 2022-10-15T13:21:39Z | |
dc.date.created | 2021-11-09T19:40:43Z | |
dc.date.issued | 2020-11 | |
dc.identifier | Pérez, Pablo Aníbal; Toledo, Jonathan; Sosa, Liliana del Valle; Peinetti, Nahuel; Torres, Alicia Ines; et al.; The phthalate DEHP modulates the estrogen receptors α and β increasing lactotroph cell population in female pituitary glands; Pergamon-Elsevier Science Ltd; Chemosphere; 258; 11-2020; 1-27 | |
dc.identifier | 0045-6535 | |
dc.identifier | http://hdl.handle.net/11336/146500 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4390821 | |
dc.description.abstract | Humans are exposed to numerous endocrine disruptors on a daily basis, which may interfere with endogenous estrogens, with Di-(2-ethylhexyl) phthalate (DEHP) being one of the most employed. The anterior pituitary gland is a target of 17β-estradiol (E2) through the specific estrogen receptors (ERs) α and β, whose expression levels fluctuate in the gland under different contexts, and the ERα/β index is responsible for the final E2 effect. The aim of the present study was to evaluate in vivo and in vitro the DEHP effects on ERα and β expression in the pituitary cell population, and also its impact on lactotroph and somatotroph cell growth. Our results revealed that perinatal exposure to DEHP altered the ERα and β expression pattern in pituitary glands from prepubertal and adult female rats and increased the percentage of lactotroph cells in adulthood. In the in vitro system, DEHP down-regulated ERα and β expression, and as a result increased the ERα/β ratio and decreased the percentages of lactotrophs and somatotrophs expressing ERα and β. In addition, DEHP increased the S + G2M phases, Ki67 index and cyclin D1 in vitro, leading to a rise in the lactotroph and somatotroph cell populations. These results showed that DEHP modified the pituitary ERα and β expression in lactotrophs and somatotrophs from female rats and had an impact on the pituitary cell growth. These changes in ER expression may be a mechanism underlying DEHP exposure in the pituitary gland, leading to cell growth deregulation. | |
dc.language | eng | |
dc.publisher | Pergamon-Elsevier Science Ltd | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0045653520314971 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.chemosphere.2020.127304 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | CELL PROLIFERATION | |
dc.subject | DEHP | |
dc.subject | ESTROGEN RECEPTOR ALPHA | |
dc.subject | ESTROGEN RECEPTOR BETA | |
dc.subject | LACTOTROPH | |
dc.subject | PITUITARY | |
dc.subject | SOMATOTROPH | |
dc.title | The phthalate DEHP modulates the estrogen receptors α and β increasing lactotroph cell population in female pituitary glands | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |