dc.creator | Maidana, Silvina Soledad | |
dc.creator | Miño, Orlando Samuel | |
dc.creator | Apóstolo, Romina María | |
dc.creator | De Stefano, Gabriel A. | |
dc.creator | Romera, Sonia | |
dc.date.accessioned | 2021-11-04T14:44:27Z | |
dc.date.accessioned | 2022-10-15T12:51:01Z | |
dc.date.available | 2021-11-04T14:44:27Z | |
dc.date.available | 2022-10-15T12:51:01Z | |
dc.date.created | 2021-11-04T14:44:27Z | |
dc.date.issued | 2020-02 | |
dc.identifier | Maidana, Silvina Soledad; Miño, Orlando Samuel; Apóstolo, Romina María; De Stefano, Gabriel A.; Romera, Sonia; A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 32; 1; 2-2020; 112-117 | |
dc.identifier | 1040-6387 | |
dc.identifier | http://hdl.handle.net/11336/145991 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4388097 | |
dc.description.abstract | Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains. | |
dc.language | eng | |
dc.publisher | American Association of Veterinary Laboratory Diagnosticians | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/1040638719898692 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/1040638719898692 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | BOHV-1 | |
dc.subject | BOHV-1 SUBTYPING | |
dc.subject | MULTIPLEX PCR | |
dc.subject | RESTRICTION ENDONUCLEASE ANALYSIS | |
dc.title | A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |