Design of affinity chromatography peptide ligands through combinatorial peptide libraries screening

dc.contributorLabrou, Nikolaos E.
dc.creatorBarredo, Gabriela Romina
dc.creatorSaavedra, Soledad Lorena
dc.creatorMartínez Ceron, María Camila
dc.creatorGiudicessi, Silvana Laura
dc.creatorMarani, Mariela Mirta
dc.date.accessioned2021-09-01T23:40:41Z
dc.date.accessioned2022-10-15T12:12:59Z
dc.date.available2021-09-01T23:40:41Z
dc.date.available2022-10-15T12:12:59Z
dc.date.created2021-09-01T23:40:41Z
dc.date.issued2020
dc.identifierBarredo, Gabriela Romina; Saavedra, Soledad Lorena; Martínez Ceron, María Camila; Giudicessi, Silvana Laura; Marani, Mariela Mirta; Design of affinity chromatography peptide ligands through combinatorial peptide libraries screening; Springer; 2178; 2020; 217-244
dc.identifier978-1-0716-0774-9
dc.identifier1064-3745
dc.identifierhttp://hdl.handle.net/11336/139472
dc.identifier1940-6029
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4384634
dc.description.abstractIn this chapter, a protocol to design affinity chromatography matrices with short peptide ligands immobilized for protein purification is described. The first step consists of the synthesis of a combinatorial peptide library on the hydroxymethylbenzoyl (HMBA)-ChemMatrix resin by the divide–couple–recombine (DCR) method using the Fmoc chemistry. Next, the library is screened with the protein of interest labeled with a fluorescent dye or biotin. Subsequently, peptides contained on positive beads are identified by tandem matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS), and those sequences showing greater consensus are synthesized in larger quantities and immobilized on chromatographic supports. Finally, target protein adsorption on peptide affinity matrices is evaluated through equilibrium adsorption isotherms and breakthrough curves.
dc.languageeng
dc.publisherSpringer
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-0716-0775-6_16#DOI
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/978-1-0716-0775-6_16#DOI
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourceProtein downstream processing: design, Development and application of high and low-resolution methods
dc.subjectPEPTIDE LIGAND
dc.subjectSOLID-PHASE-PEPTIDE SYNTHESIS
dc.subjectONE-BEAD-ONE-PEPTIDE
dc.subjectCOMBINATORIAL LIBRARIE
dc.subjectCHEMMATRIX RESIN
dc.subjectTANDEM-MASS SPECTROMETRY
dc.titleDesign of affinity chromatography peptide ligands through combinatorial peptide libraries screening
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeinfo:eu-repo/semantics/bookPart
dc.typeinfo:ar-repo/semantics/parte de libro


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