dc.creator | Minnaard, Jessica | |
dc.creator | Lievin Le Moal, Vanessa | |
dc.creator | Coconnier, Marie Helene | |
dc.creator | Servin, Alain L. | |
dc.creator | Perez, Pablo Fernando | |
dc.date.accessioned | 2022-05-27T12:39:07Z | |
dc.date.accessioned | 2022-10-15T11:39:35Z | |
dc.date.available | 2022-05-27T12:39:07Z | |
dc.date.available | 2022-10-15T11:39:35Z | |
dc.date.created | 2022-05-27T12:39:07Z | |
dc.date.issued | 2004-06 | |
dc.identifier | Minnaard, Jessica; Lievin Le Moal, Vanessa; Coconnier, Marie Helene; Servin, Alain L.; Perez, Pablo Fernando; Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells; American Society for Microbiology; Infection and Immunity; 72; 6; 6-2004; 3106-3112 | |
dc.identifier | 0019-9567 | |
dc.identifier | http://hdl.handle.net/11336/158334 | |
dc.identifier | 1098-5522 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4381853 | |
dc.description.abstract | In the present study the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus has been investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with B. cereus in exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, three hours-old cultures of some strains completely detached the cultured cells after 3 h post-infection, whereas no such effect was found with 16 h-old cultures. Infection of enterocyte-like cells with B. cereus leads to disruption of the F-actin network and necrosis. Even though, the effect of secreted factors cannot be ruled out, the direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some B. cereus strains were able to internalize into Caco-2 cells. Our findings add a new insight on the mechanisms of virulence of B. cereus in the context of intestinal infection. | |
dc.language | eng | |
dc.publisher | American Society for Microbiology | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/IAI.72.6.3106-3112.2004 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/IAI.72.6.3106-3112.2004 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Bacillus cereus | |
dc.subject | Human Intestinal | |
dc.title | Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |