dc.creatorFigueroa, Carlos Maria
dc.creatorAsención Diez, Matías Damián
dc.creatorKuhn, Misty L.
dc.creatorMcEwen, Sheila
dc.creatorSalerno, Graciela Lidia
dc.creatorIglesias, Alberto Alvaro
dc.creatorBallicora, Miguel A.
dc.date.accessioned2019-06-13T20:01:06Z
dc.date.accessioned2022-10-15T11:35:52Z
dc.date.available2019-06-13T20:01:06Z
dc.date.available2022-10-15T11:35:52Z
dc.date.created2019-06-13T20:01:06Z
dc.date.issued2013-01
dc.identifierFigueroa, Carlos Maria; Asención Diez, Matías Damián; Kuhn, Misty L.; McEwen, Sheila; Salerno, Graciela Lidia; et al.; The unique nucleotide specificity of the sucrose synthase from Thermosynechococcus elongatus; Elsevier Science; FEBS Letters; 587; 2; 1-2013; 165-169
dc.identifier0014-5793
dc.identifierhttp://hdl.handle.net/11336/78276
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4381498
dc.description.abstractSucrose synthase catalyzes the reversible conversion of sucrose and UDP into fructose and UDP-glucose. In filamentous cyanobacteria, the sucrose cleavage direction plays a key physiological function in carbon metabolism, nitrogen fixation, and stress tolerance. In unicellular strains, the function of sucrose synthase has not been elucidated. We report a detailed biochemical characterization of sucrose synthase from Thermosynechococcus elongatus after the gene was artificially synthesized for optimal expression in Escherichia coli. The homogeneous recombinant sucrose synthase was highly specific for ADP as substrate, constituting the first one with this unique characteristic, and strongly suggesting an interaction between sucrose and glycogen metabolism.
dc.languageeng
dc.publisherElsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.febslet.2012.11.011
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.febslet.2012.11.011
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCarbohydrate Metabolism
dc.subjectCyanobacteria
dc.subjectGlycogen Synthesis
dc.subjectSucrose Synthase
dc.titleThe unique nucleotide specificity of the sucrose synthase from Thermosynechococcus elongatus
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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