dc.creator | Sosa, Gustavo Adolfo | |
dc.creator | Quiroga, María Florencia | |
dc.creator | Roux, Maria | |
dc.date.accessioned | 2020-09-10T20:59:48Z | |
dc.date.accessioned | 2022-10-15T11:23:29Z | |
dc.date.available | 2020-09-10T20:59:48Z | |
dc.date.available | 2022-10-15T11:23:29Z | |
dc.date.created | 2020-09-10T20:59:48Z | |
dc.date.issued | 2009-05 | |
dc.identifier | Sosa, Gustavo Adolfo; Quiroga, María Florencia; Roux, Maria; Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats; Elsevier Gmbh; Immunobiology; 214; 5; 5-2009; 384-391 | |
dc.identifier | 0171-2985 | |
dc.identifier | http://hdl.handle.net/11336/113752 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4380426 | |
dc.description.abstract | Nasopharynx-associated lymphoid tissue (NALT) is responsible for immune responses in the upper respiratory tract of rodents. In our model of protein malnutrition (R21 group), bronchus-associated lymphoid tissue (BALT), situated in the lower respiratory tract, showed a decrease of CD4+, CD8α+, and TCRαβ+ lymphocytes but TCRγδ+ cells were increased. Besides, there is no information regarding the frequencies of T-cell populations in 60-day-old Wistar rats (C60 group). So, the aim of the present study was to analyze by flow cytometry NALT T-cells from both groups. NALT lymphocytes were isolated from R21 and C60 groups and stained with different antibodies. Samples were run on a FACScalibur flow cytometer. Background staining was evaluated using isotype controls. Data analysis was performed using BD Cell Quest® and WinMDI 2.9. In C60, the predominant population was CD4+TCRαβ+, which was significantly diminished in the R21 group. However, CD8α+, the majority expressing CD8αβ, and TCRγδ+ cells were not affected. In our model of secondary immunodeficiency, there is a compartmentalization between NALT and BALT because they differ in the populations affected even though they are inductive sites of the respiratory tract in the common mucosal immune system. | |
dc.language | eng | |
dc.publisher | Elsevier Gmbh | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0171298508001332 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.imbio.2008.10.005 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | Malnutrition; Wistar rats | |
dc.subject | NALT; | |
dc.subject | TCRab | |
dc.subject | Secondary immunodeficiency | |
dc.subject | CD4 | |
dc.subject | TCRgd | |
dc.title | Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |