dc.creator | Ramhorst, Rosanna Elizabeth | |
dc.creator | Fraccaroli, Laura Virginia | |
dc.creator | Aldo, Paulomi | |
dc.creator | Alvero, Ayesha B. | |
dc.creator | Cardenas, Ingrid | |
dc.creator | Perez Leiros, Claudia | |
dc.creator | Mor, Gil | |
dc.date.accessioned | 2018-12-18T17:38:14Z | |
dc.date.accessioned | 2022-10-15T11:05:00Z | |
dc.date.available | 2018-12-18T17:38:14Z | |
dc.date.available | 2022-10-15T11:05:00Z | |
dc.date.created | 2018-12-18T17:38:14Z | |
dc.date.issued | 2012-01 | |
dc.identifier | Ramhorst, Rosanna Elizabeth; Fraccaroli, Laura Virginia; Aldo, Paulomi; Alvero, Ayesha B.; Cardenas, Ingrid; et al.; Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells; Wiley Blackwell Publishing, Inc; American Journal of Reproductive Immunology; 67; 1; 1-2012; 17-27 | |
dc.identifier | 1046-7408 | |
dc.identifier | http://hdl.handle.net/11336/66667 | |
dc.identifier | 8755-8920 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4378814 | |
dc.description.abstract | Problem The specialized regulatory T-cells (Treg) population, essential for maternal tolerance of the fetus, performs its suppressive actions in the critical peri-implantation phase of pregnancy. In the present work, we investigated whether trophoblast cells are able to induce Treg recruitment, differentiation, and whether these mechanisms are modified by a bacterial or viral infection. Method of Study Human T-regulatory cells were differentiated from naïve CD45RA + CCR7 + cells obtained from peripheral blood mononuclear cells cultured with IL-2 and TGFβ over 5days. Induction of iTregs (CD4 +Foxp3 + cells) was evaluated using low serum conditioned media (LSCM), obtained from two first trimester trophoblast cell lines, Swan-71 and HTR8. Coculture experiments were carried out using transwell assays where trophoblast cells were in the absence or presence of PGN, LPS, or Poly [I:C]. Cytokine production was measured by multiplex analysis. Results Trophoblast cells constitutively secrete high levels of TGFβ and induced a significant increase of Foxp3 expression accompanied by a specific T-reg cytokine profile. Moreover, trophoblast cells were able to recruit iTregs in a specific manner. Conclusion We demonstrate that trophoblast cells have an active role on the recruitment and differentiation of iTregs, therefore, contributing to the process of immune regulation at the placental-maternal interface. © 2011 John Wiley & Sons A/S. | |
dc.language | eng | |
dc.publisher | Wiley Blackwell Publishing, Inc | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1111/j.1600-0897.2011.01056.x | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0897.2011.01056.x | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703637/ | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Early Pregnancy | |
dc.subject | Human Implantation | |
dc.subject | Regulatory T Cells | |
dc.subject | Tolerance And Pregnancy | |
dc.title | Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |