dc.creatorMaldonado, Lucas Luciano
dc.creatorArrabal, Juan Pablo
dc.creatorRosenzvit, Mara Cecilia
dc.creatorDe Oliveira, Guilherme Corrêa
dc.creatorKamenetzky, Laura
dc.date.accessioned2021-04-16T16:56:20Z
dc.date.accessioned2022-10-15T10:39:42Z
dc.date.available2021-04-16T16:56:20Z
dc.date.available2022-10-15T10:39:42Z
dc.date.created2021-04-16T16:56:20Z
dc.date.issued2019-08
dc.identifierMaldonado, Lucas Luciano; Arrabal, Juan Pablo; Rosenzvit, Mara Cecilia; De Oliveira, Guilherme Corrêa; Kamenetzky, Laura; Revisiting the phylogenetic history of helminths through genomics, the case of the new echinococcus oligarthrus genome; Frontiers Media S.A.; Frontiers in Genetics; 10; JUL; 8-2019; 1-13
dc.identifier1664-8021
dc.identifierhttp://hdl.handle.net/11336/130223
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4376605
dc.description.abstractThe first parasitic helminth genome sequence was published in 2007; since then, only ~200 genomes have become available, most of them being draft assemblies. Nevertheless, despite the medical and economical global impact of helminthic infections, parasite genomes in public databases are underrepresented. Recently, through an integrative approach involving morphological, genetic, and ecological aspects, we have demonstrated that the complete life cycle of Echinococcus oligarthrus (Cestoda: Taeniidae) is present in South America. The neotropical E. oligarthrus parasite is capable of developing in any felid species and producing human infections. Neotropical echinococcosis is poorly understood yet and requires a complex medical examination to provide the appropriate intervention. Only a few cases of echinococcosis have been unequivocally identified and reported as a consequence of E. oligarthrus infections. Regarding phylogenetics, the analyses of mitogenomes and nuclear datasets have resulted in discordant topologies, and there is no unequivocal taxonomic classification of Echinococcus species so far. In this work, we sequenced and assembled the genome of E. oligarthrus that was isolated from agoutis (Dasyprocta azarae) naturally infected and performed the first comparative genomic study of a neotropical Echinococcus species. The E. oligarthrus genome assembly consisted of 86.22 Mb which showed ~90% identity and 76.3% coverage with Echinococcus multilocularis and contained the 85.0% of the total expected genes. Genetic variants analysis of whole genome revealed a higher rate of intraspecific genetic variability (23,301 SNPs; 0.22 SNPs/kb) rather than for the genomes of E. multilocularis and Echinococcus canadensis G7 but lower with respect to Echinococcus granulosus G1. Comparative genomics against E. multilocularis, E. granulosus G1, and E. canadensis G7 revealed 38,762, 125,147, and 170,049 homozygous polymorphic sites, respectively, indicating a higher genetic distance between E. oligarthrus and E. granulosus sensu lato species. The SNP distribution in chromosomes revealed a higher SNP density in the longest chromosomes. Phylogenetic analysis using whole-genome SNPs demonstrated that E. oligarthrus is one of the basal species of the genus Echinococcus and is phylogenetically closer to E. multilocularis. This work sheds light on the Echinococcus phylogeny and settles the basis to study sylvatic Echinococcus species and their developmental evolutionary features.
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fgene.2019.00708/full
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fgene.2019.00708
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCHROMOSOMES
dc.subjectECHINOCOCCUS OLIGARTHRUS
dc.subjectGENOME
dc.subjectPARASITES
dc.subjectPHYLOGENY
dc.subjectSINGLE NUCLEOTIDE POLYMORPHISM
dc.titleRevisiting the phylogenetic history of helminths through genomics, the case of the new echinococcus oligarthrus genome
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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