dc.creatorReppetti, Julieta
dc.creatorReca, Alejandra Daniela
dc.creatorSeyahian, Erika Abril
dc.creatorMedina Mora, Yollyseth Astrid
dc.creatorMartinez, Nora Alicia
dc.creatorSzpilbarg, Natalia
dc.creatorDamiano, Alicia Ermelinda
dc.date.accessioned2020-12-17T13:25:05Z
dc.date.accessioned2022-10-15T10:39:17Z
dc.date.available2020-12-17T13:25:05Z
dc.date.available2022-10-15T10:39:17Z
dc.date.created2020-12-17T13:25:05Z
dc.date.issued2019-09
dc.identifierReppetti, Julieta; Reca, Alejandra Daniela; Seyahian, Erika Abril; Medina Mora, Yollyseth Astrid; Martinez, Nora Alicia; et al.; Intact caveolae are required for proper extravillous trophoblast migration and differentiation; Veterinary and Human Toxicology; Journal of Cellular Physiology; 235; 4; 9-2019; 3382-3392
dc.identifier0021-9541
dc.identifierhttp://hdl.handle.net/11336/120722
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4376562
dc.description.abstractCaveolae constitute membrane domains critical for the organization and synchronization of different signaling molecules related to numerous cell processes such as cell migration, invasion, and differentiation. Caveolin-1 (Cav-1) is the main integral membrane protein of these domains. Recently, it was found that a normal expression of aquaporin-3 (AQP3) is required for extravillous trophoblast (EVT) cell migration. Our aim was to investigate the role of caveolae in the migration, invasion, and endovascular differentiation of human EVT cells during placentation and its interaction with AQP3. EVT cells (Swan 71 cell line) were cultured in complete Dulbecco's modified Eagle's medium–nutrient mixture F12 and treated with 5 mM methyl-β-cyclodextrin (MβCD) to disrupt caveolae. We found that after MβCD treatment, Cav-1 protein was undetectable. In this condition, the ability of the cells to migrate was significantly decreased compared with the control cells, while no differences were observed in the number of invading cells and the metalloproteinases activity between control and MβCD-treated cells. Surprisingly, the disruption of caveolae significantly enhanced EVT endovascular differentiation. On the contrary, the silencing of AQP3, negatively affected tube-like formation. The theoretical analysis of the primary sequence of AQP3 protein revealed a putative Cav-1-binding site. In addition, immunoprecipitation and double immunofluorescence assays showed that AQP3 colocalized with Cav-1. These results showed that during placentation an intact caveola in EVT cells may be necessary for AQP3 and Cav-1 interaction and any perturbations might result in serious pregnancy disorders.
dc.languageeng
dc.publisherVeterinary and Human Toxicology
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29226
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jcp.29226
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAQP3
dc.subjectCAVEOLAE
dc.subjectPLACENTATION
dc.subjectTROPHOBLAST
dc.titleIntact caveolae are required for proper extravillous trophoblast migration and differentiation
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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