dc.creator | Canepa, Gaspar Exequiel | |
dc.creator | Bouvier, León Alberto | |
dc.creator | Miranda, Mariana Reneé | |
dc.creator | Uttaro, Antonio Domingo | |
dc.creator | Pereira, Claudio Alejandro | |
dc.date.accessioned | 2020-04-24T19:46:11Z | |
dc.date.accessioned | 2022-10-15T10:21:59Z | |
dc.date.available | 2020-04-24T19:46:11Z | |
dc.date.available | 2022-10-15T10:21:59Z | |
dc.date.created | 2020-04-24T19:46:11Z | |
dc.date.issued | 2009-03 | |
dc.identifier | Canepa, Gaspar Exequiel; Bouvier, León Alberto; Miranda, Mariana Reneé; Uttaro, Antonio Domingo; Pereira, Claudio Alejandro; Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 292; 1; 3-2009; 27-32 | |
dc.identifier | 0378-1097 | |
dc.identifier | http://hdl.handle.net/11336/103590 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4375091 | |
dc.description.abstract | L-Cysteine and methionine are unique amino acids that act as sulfur donors in all organisms. In the specific case of Trypanosomatids, L-cysteine is particularly relevant as a substrate in the synthesis of trypanothione. Although it can be synthesized de novo, L-cysteine is actively transported in Trypanosoma cruzi epimastigote cells. L-Cysteine uptake is highly specific; none of the amino acids assayed yield significant differences in terms of transport rates. L-Cysteine is transported by epimastigote cells with a calculated apparent Km of 49.5 μM and a Vmax of about 13 pmol min−1 per 107 cells. This transport is finely regulated by amino acid starvation, extracellular pH, and between the parasite growth phases. In addition, L-cysteine is incorporated post-translationally into proteins, suggesting its role in iron–sulfur core formation. Finally, the metabolic fates of L-cysteine were predicted in silico. | |
dc.language | eng | |
dc.publisher | Wiley Blackwell Publishing, Inc | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1574-6968.2008.01467.x | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/292/1/27/490065 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | Trypanosoma cruzi | |
dc.subject | metabolite transport | |
dc.subject | L-cysteine | |
dc.subject | amino acid transporter | |
dc.subject | Chagas disease | |
dc.title | Characterization of Trypanosoma cruzi l-cysteine transport mechanisms and their adaptive regulation | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |