dc.creator | Rivero, Cintia Wanda | |
dc.creator | Garcia, Natalia Soledad | |
dc.creator | Fernández Lucas, Jesús | |
dc.creator | Betancor, Lorena | |
dc.creator | Romanelli, Gustavo Pablo | |
dc.creator | Trelles, Jorge Abel | |
dc.date.accessioned | 2022-08-17T16:08:33Z | |
dc.date.accessioned | 2022-10-15T10:06:58Z | |
dc.date.available | 2022-08-17T16:08:33Z | |
dc.date.available | 2022-10-15T10:06:58Z | |
dc.date.created | 2022-08-17T16:08:33Z | |
dc.date.issued | 2021-04 | |
dc.identifier | Rivero, Cintia Wanda; Garcia, Natalia Soledad; Fernández Lucas, Jesús; Betancor, Lorena; Romanelli, Gustavo Pablo; et al.; Green production of cladribine by using immobilized 2′-deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology; MDPI AG; Biomolecules; 11; 5; 4-2021; 657-660 | |
dc.identifier | 2218-273X | |
dc.identifier | http://hdl.handle.net/11336/165857 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4373794 | |
dc.description.abstract | Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60◦ C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale. | |
dc.language | eng | |
dc.publisher | MDPI AG | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-273X/11/5/657 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/biom11050657 | |
dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | ANTINEOPLASTIC DRUG | |
dc.subject | BIOMIMETIC SILICA | |
dc.subject | CALCIUM ALGINATE | |
dc.subject | ENTRAPMENT | |
dc.subject | ENZYME IMMOBILIZATION | |
dc.subject | GLUTARALDEHYDE | |
dc.title | Green production of cladribine by using immobilized 2′-deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |