dc.creator | Castillo, Graciela | |
dc.creator | Chanampa, Yolanda | |
dc.creator | Orce, Gabriel Guillermo F. | |
dc.date.accessioned | 2020-01-17T20:07:36Z | |
dc.date.accessioned | 2022-10-15T09:50:52Z | |
dc.date.available | 2020-01-17T20:07:36Z | |
dc.date.available | 2022-10-15T09:50:52Z | |
dc.date.created | 2020-01-17T20:07:36Z | |
dc.date.issued | 2005-12 | |
dc.identifier | Castillo, Graciela; Chanampa, Yolanda; Orce, Gabriel Guillermo F.; Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 140; 1; 12-2005; 21-27 | |
dc.identifier | 0742-8413 | |
dc.identifier | http://hdl.handle.net/11336/95101 | |
dc.identifier | 1532-0456 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4372446 | |
dc.description.abstract | The amphibian skin, widely used for studying the transepithelial passage of electrolytes, exhibits anion pathways relatively specific for Cl-. We studied the effect of HgCl2, 1.0×10-4 M on its electrical parameters and unidirectional anion fluxes. In the presence of Cl-, the transepithelial conductance (G) of the isolated skin of the Bufo arenarum toad increased considerably following exposure to HgCl 2, whereas short-circuit current (SCC)-reflecting transepithelial Na+ transport-underwent only slight stimulation. Following the blockade of Na+ intake by amiloride, 1.0×10-4 M, the removal of Cl- from the solution bathing the epidermal border of the skin brought about a decrease in G, and gave rise to a gradient-induced SCC (SCCg) consistent with transepithelial passage of Cl- along its gradient. Addition of mercaptoethanol, 5.0×10-3 M to the bath containing Hg2+ fully reversed these effects. The increase in G was accompanied by an increase in the unidirectional (epidermal to dermal) fluxes of 36Cl- and 131I-, and a decrease in the passage of 99mTcO4-. These results show the effects of HgCl2 to be similar to those of theophylline, although exhibiting a different selectivity. Our data suggest that anion passage following exposure to HgCl2 is, like that stimulated by theophylline, predominantly if not exclusively transcellular, and does not involve a significant opening of the tight junctions. | |
dc.language | eng | |
dc.publisher | Elsevier Science Inc | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.cca.2004.12.003 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045604002509 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | 131I - | |
dc.subject | 36CL- | |
dc.subject | 99MTCO4- | |
dc.subject | BUFO ARENARUM | |
dc.subject | EPITHELIAL ANION PERMEABILITY | |
dc.subject | SHORT-CIRCUIT CURRENT | |
dc.subject | TOAD SKIN | |
dc.subject | TRANSEPITHELIAL CONDUCTANCE | |
dc.subject | UNIDIRECTIONAL FLUXES | |
dc.title | Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |