dc.creatorde la Mora de la Mora, José Ignacio
dc.creatorEnríquez Flores, Sergio
dc.creatorFernández Lainez, Cynthia
dc.creatorGutiérrez Castrellón, Pedro
dc.creatorOlivos García, Alfonso
dc.creatorGonzález Canto, Augusto
dc.creatorHernández, Roberto
dc.creatorLujan, Hugo Daniel
dc.creatorGarcía Torres, Itzhel
dc.creatorLópez Velázquez, Gabriel
dc.date.accessioned2020-12-17T20:35:57Z
dc.date.accessioned2022-10-15T09:44:02Z
dc.date.available2020-12-17T20:35:57Z
dc.date.available2022-10-15T09:44:02Z
dc.date.created2020-12-17T20:35:57Z
dc.date.issued2019-01
dc.identifierde la Mora de la Mora, José Ignacio; Enríquez Flores, Sergio; Fernández Lainez, Cynthia; Gutiérrez Castrellón, Pedro; Olivos García, Alfonso; et al.; Characterization of proteolytic activities of Giardia lamblia with the ability to cleave His-tagged N-terminal sequences; Elsevier Science; Molecular and Biochemical Parasitology; 228; 1-2019; 16-26
dc.identifier0166-6851
dc.identifierhttp://hdl.handle.net/11336/120798
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4371849
dc.description.abstractGiardia lamblia is one of the most common protozoan infectious agents in the world and is responsible for diarrheal disease and chronic postinfectious illness. During the host-parasite interaction, proteases are important molecules related to virulence, invasion, and colonization, not only for Giardia but also for other parasites. We aimed to characterize the cysteine protease activity detected in trophozoite lysates. This proteolytic activity showed the ability to cleave NH-terminal sequences with either a recognition sequence for a viral protease or a recognition sequence for thrombin. This cleavage activity was detected in nonencysting trophozoites and increased with the progression of encystation. This activity was also detected in excretion/secretion products of axenic trophozoites and in trophozoites cocultured with differentiated Caco-2 cells. Based on size exclusion chromatography, we obtained a fraction enriched in low- to medium-molecular-weight proteins that was capable of exerting this cleavage activity and aggregating human platelets. Finally, our results suggest that this proteolytic activity is shared with other protozoan parasites.
dc.languageeng
dc.publisherElsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.molbiopara.2019.01.001
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166685118301695?via%3Dihub
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCATHEPSIN
dc.subjectCYSTEINE PROTEASE
dc.subjectGIARDIASIS
dc.subjectPAR
dc.subjectTRIOSEPHOSPHATE ISOMERASE
dc.titleCharacterization of proteolytic activities of Giardia lamblia with the ability to cleave His-tagged N-terminal sequences
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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