dc.creator | Rodríguez, María Celeste | |
dc.creator | Attallah, Carolina Veronica | |
dc.creator | Lozano, Victoria | |
dc.creator | Etcheverrigaray, Marina | |
dc.creator | Oggero Eberhardt, Marcos Rafael | |
dc.date.accessioned | 2022-02-14T22:01:59Z | |
dc.date.accessioned | 2022-10-15T09:23:41Z | |
dc.date.available | 2022-02-14T22:01:59Z | |
dc.date.available | 2022-10-15T09:23:41Z | |
dc.date.created | 2022-02-14T22:01:59Z | |
dc.date.issued | 2019-11 | |
dc.identifier | Rodríguez, María Celeste; Attallah, Carolina Veronica; Lozano, Victoria; Etcheverrigaray, Marina; Oggero Eberhardt, Marcos Rafael; Design and validation of an immuno-PCR assay for IFN-α2b quantification in human plasma; Future Medicine; Bioanalysis; 11; 23; 11-2019; 2175-2188 | |
dc.identifier | 1757-6180 | |
dc.identifier | http://hdl.handle.net/11336/151994 | |
dc.identifier | 1757-6199 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4370065 | |
dc.description.abstract | Aim: Nowadays, IFN-α is considered a promising therapeutic target for systemic lupus erythematosus. An immuno-PCR (iPCR) was developed to quantify low amounts of IFN-α in human plasma followed by a deep analysis of the methodologic robustness throughout quality by design approach. Results: An accurate, sensitive, selective and versatile iPCR was validated. The critical iPCR procedural steps were identified, applying a Plackett-Burman design. Also, this assay demonstrated an outstanding LOD of 0.3 pg/ml. A significant aspect relies on its high versatility to detect and quantify other cytokines in human plasma as the appropriate biotinylated antibody is employed. Conclusion: This reliable iPCR assay can be clinically used as an alternative method for quantitating and detecting low IFN-α2b concentrations in human plasma samples. | |
dc.language | eng | |
dc.publisher | Future Medicine | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.future-science.com/doi/10.4155/bio-2019-0225 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4155/bio-2019-0225 | |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | INF-ALPHA | |
dc.subject | IMMUNO-PCR | |
dc.subject | QUALITY BY DESIGN | |
dc.subject | ELISA | |
dc.subject | ROBUSTNESS | |
dc.subject | ACCURANCY | |
dc.subject | PRECISION | |
dc.subject | SELECTIVITY | |
dc.subject | LINEAR RANGE | |
dc.subject | SENSITIVITY | |
dc.title | Design and validation of an immuno-PCR assay for IFN-α2b quantification in human plasma | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |