Methodological aspects of Universal immuno-PCR on standard tubes

dc.creatorAbud, Julián Elías
dc.creatorSantamaría, Clarisa Guillermina
dc.creatorOggero Eberhardt, Marcos Rafael
dc.creatorRodriguez, Horacio Adolfo
dc.date.accessioned2020-05-08T13:00:04Z
dc.date.accessioned2022-10-15T08:23:15Z
dc.date.available2020-05-08T13:00:04Z
dc.date.available2022-10-15T08:23:15Z
dc.date.created2020-05-08T13:00:04Z
dc.date.issued2019-02
dc.identifierAbud, Julián Elías; Santamaría, Clarisa Guillermina; Oggero Eberhardt, Marcos Rafael; Rodriguez, Horacio Adolfo; Methodological aspects of Universal immuno-PCR on standard tubes; Academic Press Inc Elsevier Science; Analytical Biochemistry; 570; 2-2019; 56-61
dc.identifier0003-2697
dc.identifierhttp://hdl.handle.net/11336/104584
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4364662
dc.description.abstractOne of the most used formats in inmuno-polymerase chain reaction (IPCR) is known as "Universal" IPCR (signal-generating complexes is based on conjugates of biotinylated DNA, biotinylated IgG and avidin). In the present study, we evaluated the utility of using mono- and bi-biotinylated DNA probes, pre-self-assembled DNA-neutravidin complex, blocking step and glutaraldehyde pretreatment of standard PCR tubes to improve the analytical performance of the hTSH-IPCR assay. The use of pre-self-assembled mono-biotinylated DNA-neutravidin complex enhances both the sensitivity and the reproducibility of the hTSH-IPCR assay, even without blocking step: hTSH-IPCR assay showed an improved limit of detection (LOD: 0.01 μIU/ml), calibration sensitivity (SEN: 2.4) and analytic sensitivity (γ: 9 μIU/ml −1 ) in comparison with both a self-made ELISA and a commercial one.
dc.languageeng
dc.publisherAcademic Press Inc Elsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0003269718312508
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ab.2019.02.007
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectDIAGNOSTIC
dc.subjectHUMAN TSH
dc.subjectIMMUNE-PCR
dc.subjectIMMUNOASSAYS
dc.subjectMONOCLONAL ANTIBODIES
dc.titleMethodological aspects of Universal immuno-PCR on standard tubes
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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