Argentina | info:eu-repo/semantics/article
dc.creatorAlmada, Juan Cruz
dc.creatorBortolotti, Ana
dc.creatorRuysschaert, Jean Marie
dc.creatorde Mendoza, Diego
dc.creatorInda, María Eugenia
dc.creatorCybulski, Larisa Estefania
dc.date.accessioned2022-03-17T03:56:10Z
dc.date.accessioned2022-10-15T08:08:09Z
dc.date.available2022-03-17T03:56:10Z
dc.date.available2022-10-15T08:08:09Z
dc.date.created2022-03-17T03:56:10Z
dc.date.issued2021-07
dc.identifierAlmada, Juan Cruz; Bortolotti, Ana; Ruysschaert, Jean Marie; de Mendoza, Diego; Inda, María Eugenia; et al.; Interhelical h-bonds modulate the activity of a polytopic transmembrane kinase; Multidisciplinary Digital Publishing Institute; Biomolecules; 11; 7; 7-2021; 1-12
dc.identifier2218-273X
dc.identifierhttp://hdl.handle.net/11336/153484
dc.identifier2218-273X
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4363583
dc.description.abstractDesK is a Histidine Kinase that allows Bacillus subtilis to maintain lipid homeostasis in response to changes in the environment. It is located in the membrane, and has five transmembrane helices and a cytoplasmic catalytic domain. The transmembrane region triggers the phosphorylation of the catalytic domain as soon as the membrane lipids rigidify. In this research, we study how transmembrane inter-helical interactions contribute to signal transmission; we designed a coexpression system that allows studying in vivo interactions between transmembrane helices. By Alanine-replacements, we identified a group of polar uncharged residues, whose side chains contain hydrogen-bond donors or acceptors, which are required for the interaction with other DesK transmembrane helices; a particular array of H-bond- residues plays a key role in signaling, transmitting information detected at the membrane level into the cell to finally trigger an adaptive response.
dc.languageeng
dc.publisherMultidisciplinary Digital Publishing Institute
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://www.mdpi.com/2218-273X/11/7/938
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/biom11070938
dc.rightshttps://creativecommons.org/licenses/by/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDIMERISATION MOTIF
dc.subjectHISTIDINE KINASE
dc.subjectHYDROGEN BOND INTERACTION
dc.subjectRECEPTOR
dc.subjectSIGNAL TRANSDUCTION
dc.subjectTRANSMEMBRANE PROTEIN INTERACTIONS
dc.titleInterhelical h-bonds modulate the activity of a polytopic transmembrane kinase
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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