dc.creatorAlvarez, Lucía Paula
dc.creatorBarbagelata, María Sol
dc.creatorGordiola, Mariana Laura
dc.creatorCheung, Ambrose L.
dc.creatorSordelli, Daniel Oscar
dc.creatorBuzzola, Fernanda Roxana
dc.date.accessioned2020-09-01T18:02:51Z
dc.date.accessioned2022-10-15T07:56:30Z
dc.date.available2020-09-01T18:02:51Z
dc.date.available2022-10-15T07:56:30Z
dc.date.created2020-09-01T18:02:51Z
dc.date.issued2010-03
dc.identifierAlvarez, Lucía Paula; Barbagelata, María Sol; Gordiola, Mariana Laura; Cheung, Ambrose L.; Sordelli, Daniel Oscar; et al.; Salicylic acid diminishes Staphylococcus aureus capsular polysaccharide type 5 expression; American Society for Microbiology; Infection and Immunity; 78; 3; 3-2010; 1339-1344
dc.identifier0019-9567
dc.identifierhttp://hdl.handle.net/11336/112914
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4362935
dc.description.abstractCapsular polysaccharides (CP) of serotypes 5 (CP5) and 8 (CP8) are major Staphylococcus aureus virulence factors. Previous studies have shown that salicylic acid (SAL), the main aspirin metabolite, affects the expression of certain bacterial virulence factors. In the present study, we found that S. aureus strain Reynolds (CP5) cultured with SAL was internalized by MAC-T cells in larger numbers than strain Reynolds organisms not exposed to SAL. Furthermore, the internalization of the isogenic nonencapsulated Reynolds strain into MAC-T cells was not significantly affected by preexposure to SAL. Pretreatment of S. aureus strain Newman with SAL also enhanced internalization into MAC-T cells compared with that of untreated control strains. Using strain Newman organisms, we evaluated the activity of the major cap5 promoter, which was significantly decreased upon preexposure to SAL. Diminished transcription of mgrA and upregulation of the saeRS transcript, both global regulators of CP expression, were found in S. aureus cultured in the presence of SAL, as ascertained by real-time PCR analysis. In addition, CP5 production by S. aureus Newman was also decreased by treatment with SAL. Collectively, our data demonstrate that exposure of encapsulated S. aureus strains to low concentrations of SAL reduced CP production, thus unmasking surface adhesins and leading to an increased capacity of staphylococci to invade epithelial cells. The high capacity of internalization of the encapsulated S. aureus strains induced by SAL pretreatment may contribute to the persistence of bacteria in certain hosts.
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://iai.asm.org/content/78/3/1339
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/IAI.00245-09
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectSTAPHYLOCOCCUS AUREUS
dc.subjectSALICYLIC ACID
dc.subjectCAPSULAR POLYSACCHARIDE
dc.titleSalicylic acid diminishes Staphylococcus aureus capsular polysaccharide type 5 expression
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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