UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography

dc.creatorBultel, Laurent
dc.creatorLandoni, Malena
dc.creatorGrand, Eric
dc.creatorCouto, Alicia Susana
dc.creatorKovensky, Jose Eduardo
dc.date.accessioned2019-01-28T17:58:30Z
dc.date.accessioned2022-10-15T07:43:21Z
dc.date.available2019-01-28T17:58:30Z
dc.date.available2022-10-15T07:43:21Z
dc.date.created2019-01-28T17:58:30Z
dc.date.issued2010-01
dc.identifierBultel, Laurent; Landoni, Malena; Grand, Eric; Couto, Alicia Susana; Kovensky, Jose Eduardo; UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography; Springer; Journal of The American Society for Mass Spectrometry; 21; 1; 1-2010; 178-190
dc.identifier1044-0305
dc.identifierhttp://hdl.handle.net/11336/68752
dc.identifier1879-1123
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4361746
dc.description.abstractNitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis.
dc.languageeng
dc.publisherSpringer
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jasms.2009.09.025
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1016%2Fj.jasms.2009.09.025
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectUV-MALDI-TOF
dc.subjectsulfated oligosaccharides
dc.titleUV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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